| OH10997 |
otIs264 III; ntIs1 V; otIs305. |
C. elegans |
otIs264 [ceh-36p::tagRFP] III. ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp-16.2p::che-1::3xHA::BLRP + rol-6(su1006)]. Rollers. Maintain at 15-20C. Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8. |
| OH11053 |
ntIs1 otIs305 otIs355 V. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp-16.2p::che-1::3xHA::BLRP + rol-6(su1006)] V. otIs355 [rab-3::tagRFP] V. Maintain at 15-20C. Rollers. Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8. |
| OH11054 |
pha-1(e2123) III; him-8(e1489) IV; ntIs1 V; otIs305; otEx4445. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp-16.2p::che-1::3xHA::BLRP + rol-6(su1006)]. otEx4445 [snb-1::NLS::RFP + pBX]. Rollers. Maintain by picking RFP+ Rollers. Maintain at 20C to maintain extrachromosomal array. Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8. |
| OH11630 |
lsy-27(ot108) II; ntIs1 V. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. 2 ASER. |
| OH14049 |
otIs629; ntIs1 V. |
C. elegans |
otIs629 [gcy-7p::TagRFP + ttx-3p::GFP]. ntIs1 [gcy-5p::GFP + lin-15(+)] V. RFP expression in ASEL neurons. GFP expression appears in ASER (in adults) and AIY neurons. Reference: Patel T & Hobert O. eLife 2017. |
| OH2871 |
che-1(ot101) I; ntIs1 V; otIs151. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V; integration of adEx1262 [gcy-5p::GFP + lin-15(+)]. otIs151 [ceh-36p::RFP + rol-6(su1006)]. Rollers. Both ASEL and ASER show GFP expression from ntIs1. |
| OH2996 |
ntIs1; lsy-2(ot64) X; otEx1777. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otEx1777 [lsy-2::GFP + rol-6(su1006)]. Maintain by picking Rollers. |
| OH3192 |
ntIs1 V. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V; integration of adEx1262 [gcy-5p::GFP + lin-15(+)]. GFP expression appears in ASER in adult animals. |
| OH7317 |
F21H12.1(ot86) rol-6(e187) II; ntIs1 V. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. Ectopic expression of ntIs1 in ASEL. Rollers. Whole genome sequenced strain. |
| OH7566 |
mgIs18 unc-24(e138) nhr-67(ot407) IV; ntIs1 V; otEx3103. |
C. elegans |
mgIs18 [ttx-3p::GFP] IV. ntIs1 [gcy-5p::GFP + lin-15(+)] V. otEx3103 [elt-2::GFP]. All worms have mgIs18 and ntIs1. mgIs18 labels AIY interneurons. ntIs1 marks one GFP+ neuron (ASER) in the head. otEx3103 [elt-2::GFP] is expressed in intestinal cells; maintain by picking animals with intestinal GFP. Worms that have lost the array are Emb, Lvl, and have an ASE defect. |
| OH7677 |
lin-59(ot104) I; lsy-12(ot563) ntIs1 V. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. Whole genome sequenced strain. |
| OH8593 |
ntIs1 V; otEx3830. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otEx3830 [ceh-36::CZ-caspase3(p17) + gcy-5::caspase3(p12)-NZ + myo-3::mCherry]. |
| OH9016 |
ntIs1 V; lsy-2(ot90) X. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. Loss of ASEL fate. 2 cells GFP+ for ASER marker. |
| OH9022 |
ntIs1 V; otIs256. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs256 [ceh-36::CZ-caspase3(p17) + gcy-7::caspase3(p12)-NZ + myo-3::mCherry]. |
| OH9639 |
ntIs1 V; him-8 IV; lim-6(ot146) X. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. 2 ASER. Him. |
| OH9846 |
otIs305 ntIs1 V. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp16-2p::che-1::3xHA::BLRP + rol-6(su1006)] V; injected as complex array with Pvu II bacterial DNA. Rollers. Rol is prone to silencing in otIs305, but hsp transgene is still active in Hobert Lab assays. otIs305 is homozygous by PCR analysis (Hobert). Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8. |
| OH9991 |
otIs114 I; otIs220 IV; lsy-12(ot563) ntIs1 V. |
C. elegans |
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs220 [gcy-5::mCherry]. otIs114 [lim-6p::GFP + rol-6(su1006)]. Rollers. 2 ASER. |