Transgene Information

NamedrSi28 View on WormBase
Description[hsf-1p::hsf-1(R145A)::GFP::unc-54utr; Cbr-unc-119(+)]

Strains carrying this transgene

Strain Genotype Species Description
OG566 drSi28 II; unc-119(ed3) III. C. elegans drSi28 [hsf-1p::hsf-1(R145A)::GFP::unc-54 3'UTR + Cbr-unc-119(+)] II. hsf-1 cDNA containing an arginine to alanine mutation at residue 145 in the DNA binding domain, with a C-terminal GFP and under control of 4 kb of the hsf-1 promoter, integrated at a single copy by MosSCI on chromosome II at ttTi5605 in EG4322. Exhibits nuclear GFP expression that redistributes into granules at a reduced rate compared to wild type immediately after 1 min 35C heat shock. Reference: Morton EA, Lamitina T. Aging Cell. 2012 Oct 26. doi: 10.1111/acel.12024.