Transgene Information

NamenIs425 View on WormBase
Description[myo-2p::GFP]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
MT19851 sptf-3(tm607)/hIn1 [unc-101(sy241)] nIs425 I; nIs175 IV. C. elegans nIs425 [myo-2p::GFP] I. nIs175 [ceh-28p::4NLS::GFP + lin-15(+)] IV. Heterozygotes are GFP+ wild type and segregate GFP+ Unc, GFP+ wild type, and GFP- sptf-3 homozygotes. nIs425 was integrated into sptf-3(tm607)/hIn1[unc-101(sy241)] I. The position of integration appears to be close to or lie within the region covered by hIn1: sptf-3(tm607) heterozygotes are GFP+ whereas sptf-3(tm607) homozygotes do not express GFP in the pharynx. Reference: Hirose T, Horvitz HR. Nature. 2013 Aug 15;500(7462):354-8.