Transgene Information

NamevtIs1 View on WormBase
Description[dat-1p::GFP + rol-6]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
MDH10 ast-1(hd1) rol-6(e187) II; ceh-43(ot406) III; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. ot406 has a dopaminergic phenotype.
MDH17 ceh-43(ot406) ceh-20(mu290) III; vtIs1 V; muEx261. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. muEx261 [ceh-20::GFP + odr-1::RFP]. Pick RFP+ animals to maintain. Rollers. ot406 has a dopaminergic phenotype. mu290 is Egl and has a PDE dopaminergic phenotype.
MDH30 ceh-43(ot406) III; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. ot406 has a dopaminergic phenotype.
MDH6 ast-1(gk463) bli-2(e768) unc-4(e120) II; vtIs1 V; norEx42. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. norEx42 [ast-1(+)(cosmid) + ttx-3::GFP + dat-1::mCherry]. Rollers. gk463 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain.
MDH60 vtIs1 V; ceh-60(ok1485) ceh-40(gk159) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers.
MDH7 ast-1(gk463) bli-2(e768) unc-4(e120) II; ceh-43(ot406) III; vtIs1 V; norEx42. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. norEx42 [ast-1(+)(cosmid) + ttx-3::GFP + dat-1::mCherry]. Rollers. ot406 has a dopaminergic phenotype. gk463 embryonic lethality is rescued by extrachromosomal array. Pick mCherry+ animals to maintain.
MDH91 ast-1(hd1) rol-6(e187) II; ceh-20(ok541) III; vtIs1 V; ceh-40(gk159) X; muEx261. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. muEx261 [ceh-20::GFP + odr-1::RFP]. Pick RFP+ animals to maintain. Rollers. Embryonic lethality of ceh-20(ok541); ceh-40(gk159) double mutants is rescued by muEx261.
MDH93 ceh-43(ot406) ceh-20(mu290) III; vtIs1 V; ceh-40(gk159) X; muEx261. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. muEx261 [ceh-20::GFP at C terminus + odr-1::RFP(su1006)]. Pick RFP+ animals to maintain. Rollers. Embryonic lethality of ceh-20(mu290); ceh-40(gk159) double mutants is rescued by muEx261. ot406 has a dopaminergic phenotype.
MDH95 ceh-20(mu290) III; vtIs1 V; ceh-40(gk159) X; muEx261. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. muEx261 [ceh-20::GFP + odr-1::RFP]. Pick RFP+ animals to maintain. Rollers. Embryonic lethality of ceh-20(mu290); ceh-40(gk159) double mutants is rescued by muEx261.
OH4041 ham-1(ot253) IV; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Missing or extra cells expressing dat-1::GFP.
OH4224 ham-1(ot257) IV; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP.
OH4226 vtIs1 V; lin-32(ot259) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH4240 lin-17(ot260) I; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. PDEs often do not express dat-1::GFP. Whole genome sequenced strain.
OH4247 vtIs1 V; dopy-6(ot263) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Missing or extra PDEs based on dat-1::GFP expression. Whole genome sequenced strain.
OH4254 vtIs1 V; vab-3(ot266) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP.
OH4265 lin-22(ot267) IV; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP in the V1 to V4 lineages.
OH4270 lin-22(ot268) IV; vtIs1V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1 in the V1 to V4 lineages. Missense mutation (L68F) affecting a conserved leucine residue in the helix-loop-helix domain.
OH4271 lin-22(ot269) IV; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)]. Rollers. Extra cells expressing dat-1::GFP in the V1 to V4 lineages.
OH4274 ztf-6(ot271) I; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Strain does not roll well, but GFP expression is easily detected. Extra cells expressing dat-1::GFP.
OH4276 ztf-6(ot273) I; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP.
OH4277 ztf-6(ot274) I; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. More cells express dat-1::GFP.
OH4299 vtIs1 V; vab-3(ot276) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP.
OH4303 ztf-6(ot280) I; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP. Whole genome sequenced strain.
OH4305 egl-1(ot282) vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP. Whole genome sequenced strain.
OH4306 dopy-5(ot283)/+ III; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Homozygous ot283 animals are 100% sterile and variable CEP phenotype. Maintain by picking single animals that segregate Sterile worms (the sterility is easy to see by eye because the sterile worms do not produce eggs or oocytes).
OH4307 dopy-5(ot284)/+ III; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Homozygous ot284 animals are 100% sterile and variable CEP phenotype. Maintain by picking single animals that segregate Sterile worms (the sterility is easy to see by eye because the sterile worms do not produce eggs or oocytes).
OH4320 lin-22(ot287) IV; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Extra cells expressing dat-1::GFP in the V1 to V4 lineages.
OH4322 ced-3(ot289) IV; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. The undead sisters of CEPvs and PVDs express dat-1::GFP.
OH4334 dopy-5(ot296)/+ III; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Homozygous ot296 animals are 100% sterile and variable CEP phenotype. Maintain by picking single animals that segregate Sterile worms (the sterility is easy to see by eye because the sterile worms do not produce eggs or oocytes).
OH4335 vtIs1 V; lin-32(ot297) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Missing CEPVs and PDEs; ADEs variably affected.
OH4339 dopy-5(ot298)/+ III; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Homozygous ot298 animals are 100% sterile and variable CEP phenotype. Maintain by picking single animals that segregate Sterile worms (the sterility is easy to see by eye because the sterile worms do not produce eggs or oocytes).
OH6071 trp-4(ot337) I; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer cells expressing dat-1::GFP. Whole genome sequenced strain.
OH6072 vtIs1 vsIs33 V; lin-32(ot338) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Missing CEPVs and PDEs; ADEs variably affected.
OH6086 ceh-43(ot345) III; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEPDs, ADEs and PDEs variably affected. Rollers.
OH6087 ceh-43(ot340) III; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Few cells expressing dat-1::GFP. Whole genome sequenced strain.
OH7007 ham-1(ot342) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH7008 vtIs1 vsIs33 V; lin-32(ot343) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH7016 ham-1(ot339) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEP vells variably affected (sometimes fewer, sometimes more cells expressing GFP).
OH7058 vtIs1 vsIs33 V; vab-3(ot346) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Extra cells expressing dat-1::GFP.
OH7095 ham-1(ot361) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEP vells variably affected (sometimes fewer, sometimes more cells expressing GFP).
OH7098 ham-1(ot364) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH7118 vtIs1 vsIs33 V; lin-32(ot366) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Missing CEPVs and PDEs; ADEs variably affected.
OH7150 ham-1(ot371) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEP vells variably affected (sometimes fewer, sometimes more cells expressing GFP).
OH7158 vtIs1 V; C52B9.2(tm413) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
OH7159 C24A1.2(tm801) III; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
OH7160 vtIs1 V; ets-5(tm866) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
OH7169 C50A2.4(tm440) IV; vtIs1 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
OH7323 ceh-43(ot406) III; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP.
OH7546 vtIs1 V; otIs198. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. otIs198 [hsp16-2::ast-1 + ttx-3::DsRed + hsp16-2::NLS::mCherry]. Rollers. Heat shock induces expression of nuclear mCherry and AST-1. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
OH8545 trp-4(ot477) I; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Whole genome sequenced strain.