Transgene Information

NamevsIs33 View on WormBase
Description[dop-3::RFP]
ReporterRFP

Strains carrying this transgene

Strain Genotype Species Description
LX811 vsIs33 V; lin-15B&lin-15A(n765) X. C. elegans vsIs33 [dop-3::RFP] V.
LX831 vsIs33 V; lin-15B&lin-15A(n765) X; vsIs28. C. elegans vsIs28 [dop-1::GFP]. vsIs33 [dop-3::RFP] V. Integration sites not known.
OH10221 ccIs4251 I; otIs77 II; ruIs37 III; jcIs1 IV; vsIs33 V. C. elegans ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. otIs77 [ttx-3p::kal-1 + unc-122p::GFP] II. ruIs37 [myo-2p::GFP + unc-119(+)] III. jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. vsIs33 [dop-3::RFP] V. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
OH10969 unc-42(ot712) vsIs33 V; otIs341 X. C. elegans vsIs33 [dop-3::RFP] V. otIs341 [mgl-1::GFP + pha-1(+)] X. unc-42(ot712) is W181=>STOP.
OH14926 bnc-1(ot721) vsIs33 V. C. elegans vsIs33 [dop-3::RFP] V. De-repression of ectopic effector genes in VA/VB class motor neurons. Reference: Kerk SY, et al. Neuron 2017 (in press).
OH6071 trp-4(ot337) I; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer cells expressing dat-1::GFP. Whole genome sequenced strain.
OH6072 vtIs1 vsIs33 V; lin-32(ot338) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Missing CEPVs and PDEs; ADEs variably affected.
OH6086 ceh-43(ot345) III; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEPDs, ADEs and PDEs variably affected. Rollers.
OH6087 ceh-43(ot340) III; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Few cells expressing dat-1::GFP. Whole genome sequenced strain.
OH7007 ham-1(ot342) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH7008 vtIs1 vsIs33 V; lin-32(ot343) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH7016 ham-1(ot339) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEP vells variably affected (sometimes fewer, sometimes more cells expressing GFP).
OH7058 vtIs1 vsIs33 V; vab-3(ot346) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Extra cells expressing dat-1::GFP.
OH7095 ham-1(ot361) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEP vells variably affected (sometimes fewer, sometimes more cells expressing GFP).
OH7098 ham-1(ot364) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP in various penetrances.
OH7118 vtIs1 vsIs33 V; lin-32(ot366) X. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Missing CEPVs and PDEs; ADEs variably affected.
OH7150 ham-1(ot371) IV; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. CEP vells variably affected (sometimes fewer, sometimes more cells expressing GFP).
OH7323 ceh-43(ot406) III; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Fewer or extra cells expressing dat-1::GFP.
OH8545 trp-4(ot477) I; vtIs1 vsIs33 V. C. elegans vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Whole genome sequenced strain.
QV65 vsIs33 V; gpIs1. C. elegans vsIs33 [dop-3::RFP] V. gpIs1 [hsp-16.2p::GFP]. Inducible GFP fluorescence after >1 hour heat shock at 35C. Reference: Leung CK, et al. PLoS One. 2013 Apr 29;8(4):e62166.
RJP56 egl-46(rp4) vsIs33 V; rpIs3. C. elegans rpIs3 [gcy-33p::GFP]. vsIs33 [dop-3::RFP] V. Loss of BAG neuron specification. egl-46(rp4) is a point mutation that causing a single amino acid change (C185Y) in the zinc finger domain of EGL-46; it behaves like a null for BAG specification phenotypes.
VP596 dvIs19 III; vsIs33 V. C. elegans dvIs19 [(pAF15) gst-4p::GFP::NLS] III. Oxidative stress-inducible GFP. vsIs33 [dop-3::RFP] V. References: Leung CK, et al. PLoS One. 2013 Apr 29;8(4):e62166. Leung CK, et al. J Vis Exp. 2011 May 19;(51).