Transgene Information

NamewleSi1582 View on WormBase
Description[unc-54::JFFluciferaseTAG158; cb-unc-119 (+)]

Strains carrying this transgene

Strain Genotype Species Description
LWA1582 wleSi1582 I. C. elegans wleSi1582 [unc-54p::JFF_luciferaseTAG185 + Cbr-unc-119(+)] I. It is likely that unc-119(ed3) remains in the background. Superficially wild-type. Expression of the luciferase reporter is dependent upon temperature-sensitive suppression of premature amber stop codon. Strain may be raised at 20C, but should be raised at 15C for several generations before assaying reporter expression. Expression of the Japanese firefly luciferase reporter can be detected using standard luciferase assays. Reference: Parrish AR, et al. ACS Chem Biol. 2012 Jul 20;7(7):1292-302.