Transgene Information

NamegeIs9 View on WormBase
Description[gpa-4p::skn-1b::GFP + rol-6(su1006)]

Strains carrying this transgene

Strain Genotype Species Description
LG345 geIs9. C. elegans geIs9 [gpa-4p::skn-1b::GFP + rol-6(su1006)]. Reference: Nature (2007) 447(7144):545-9.
LG348 skn-1(zu135) IV/nT1 [qIs51] (IV;V); geIs9. C. elegans geIs9 [gpa-4p::skn-1b::GFP + rol-6(su1006)]. Rollers. Heterozygotes are rollers with pharyngeal GFP signal, and segregate GFP+ rollers, arrested nT1[qIs51] aneuploids, and non-GFP skn-1 homozygotes (early arrest). Homozygous nT1[qIs51] inviable. Pick GFP+ rollers and check for correct segregation of progeny to maintain. skn-1 mutants are maternal-effect lethal and must be maintained as balanced heterozygotes. Reference: Bishop & Guarente, Nature (2007) 447(7144):545-9.