Transgene Information

NamewIs78 View on WormBase
Description[SCMp::GFP + ajm-1p::GFP + F58E10 (cosmid) + unc-119(+)]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
CB7248 dpy-18(e499)/subs-4(e3026) III; wIs78 IV. C.elegans wIs78 [SCMp::GFP + ajm-1p::GFP + F58E10 (cosmid) + unc-119(+)] IV. Heterozygous strain. Wild-type hermaphrodites segregating wild-type, Dpy-18, and dead eggs (subs-4 homozygotes). Pick wild-type to maintain. Reference: Gravato-Nobre et al (in preparation).
JR1000 ced-1(e1735) I; unc-119(ed4) III; wIs78 IV. C. elegans wIs78 [SCMp::GFP + ajm-1p::GFP + F58E10 (cosmid) + unc-119(+)] IV. GFP expression in seam cells and adherens junctions. ajm-1 previously called jam-1.
RG733 wIs78 IV. C. elegans wIs78 [SCMp::GFP + ajm-1p::GFP + F58E10 (cosmid) + unc-119(+)] IV. GFP expression in seam cells and adherens junctions. Check periodically for GFP in seam cells to retain robust expression. ajm-1 previously called jam-1. Derived by outcrossing JR1000. It is not know whether the ced-1(e1735) or unc-119(ed4) mutations are still present, as the array rescues both phenotypes. wIs78 males do not mate very well; heterozygotes mate fine.