Caenorhabditis Genetics Center (CGC)

Transgene Information

NameorIs1 View on WormBase
Description[unc-119(+); pie-1 promoter::GFP::MEI-1 fusion]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
EU1065 unc-119(ed3) III; orIs1. C. elegans Appears WT. orIs1 [pie-1p::GFP::mei-1 + unc-119(+)]. Maintain at 20C or above. Below 20C the GFP signal turns off.
EU2866 ltIs37 IV; orIs1. C. elegans ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. orIs1 [pie-1p::GFP::mei-1 + unc-119(+)]. Maintain at 25C to retain transgene fluorescence. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499
JH3011 cdc-37(ax2001) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3012 plk-1(ax2002) unc-119(ed3) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3013 unc-119(ed3) orIs1 III; mbk-2(dd5ax2004) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3075 tat-4(ax2009) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3076 unc-119(ed3) such-1(ax2010) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3078 apc-1(ax2012) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Formerly known as mat-2. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3080 fzy-1(ax2014) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3086 emb-30(ax2003) unc-119(ed3) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.
JH3087 xpo-2(ax2013) I; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. C. elegans orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41.