Transgene Information

NamejuIs76 View on WormBase
Description[unc-25p::GFP + lin-15(+)]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
AMH30 juIs76 II; daf-2(e1370) III. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive.
AMH31 juIs76 II; unc-33(e1193) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc; almost paralyzed. Growth slow.
AMH32 juIs76 II; unc-33(mn407) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH34 juIs76 II; unc-33(e204) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH36 juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C.
AMH38 juIs76 II; daf-2(e1370) III; unc-33(e204) IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
AMH46 juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. C. elegans Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow.
AMH50 juIs76; bec-1(ok691) IV/nT1 [qIs51] (IV;V). C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.  Heterozygotes are wild-type with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-pharyngeal GFP bec-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
CZ13799 juIs76 II. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. GFP expression in GABAergic motor neurons. [NOTE: (10/11/2018) CZ13799 was sent as a replacement for CZ1200, which was found to carry background mutations affecting neuron morphology.] Derived by outcrossing CZ1200 to remove lin-15(n765) and unidentified background mutations. Reference (for original juIs76 strain): Huang X, et al. Neuron. 2002 May 16;34(4):563-76.
CZ1632 juIs76 II; max-1(ju39) V. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Very mild Unc.
CZ1758 juIs76 II; max-1(ju142) V. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Very mild Unc.
CZ1931 juIs76 II; unc-71(ju156) III. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc.
CZ1935 juIs76 II; unc-71(ju160) III. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc.
LE2531 unc-40(n324) I; juIs76 II. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42.
LE3091 juIs76 II; unc-6(e78) X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42.
LE3190 tiam-1(tm1556) I; juIs76 II; mig-2(mu28) lqIs2 X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE3191 tiam-1(tm1556) I; juIs76 II; ced-10(n1993) lqIs3 IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE3192 tiam-1(ok772) I; juIs76 II; mig-2(mu28) lqIs2 X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE3193 tiam-1(ok772) I; juIs76 II; ced-10(n1993) lqIs3 IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE479 juIs76 II; ced-10(n1993) lqIs3 IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. Cell engulfment defect at 2-fold stage. Reference: Demarco RS & Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215.
OH4121 juIs76 II; wrk-1(ok695) X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.
OH4128 juIs76 II; evIs82b IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. evIs82b [unc-129::GFP + dpy-20(+)] IV.
XE1158 juIs76 II; wpIs15 X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. GFP expression in GABAergic motor neurons. wpIs15 [unc-47p::KillerRed] X. Slight Unc. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpIs15 produces a Shrinker phenotype after illumination by white light for 2 hrs. Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.