Caenorhabditis Genetics Center (CGC)

Transgene Information

NamezdIs5 View on WormBase
Description[mec-4p::GFP + lin-15(+)]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
BXN653 scrm-1(cjn26) zdIs5 I. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. mec-4::GFP is expressed in touch neurons. Null allele of scrm-1: 2501 bp deletion and 7 bp insertion (CACACAT) removes the entire scrm-1 coding sequence (position 11688933 - 11691433 of chromosome I).
CZ10175 zdIs5 I. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. SK4005 was outcrossed 10x to produce this strain.
HS1680 lin-44(n1792) zdIs5 I; cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); mom-2(ne874) V/nT1. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. Homozygous nT1[qIs51] are inviable. mec-4::GFP is expressed in touch neurons. Heterozygotes are strong Egl Psa GFP+ and segregate dead eggs and non-GFP Unc Egl Psa that give only dead eggs at 25C.
IC136 zdIs5 I; vab-1(dx31) II. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. Animals contain GFP expressed in the touch neurons and have a notched head phenotype.
IC156 zdIs5 I; quIs4. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. quIs4 [mec-4::myr::vab-1(G912E) + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons.
IC400 zdIs5 I; quIs5 II; him-5(e1490) V. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. quIs5 [mec-4::myr::vab-1 + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons.
NG4370 zdIs5 I; pig-1(gm344) IV. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I.
QW1075 lite-1(ce314) X; zdIs5; zfEx416. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. zfEx416 [rig-3p::GFP::SL2::mCherry]. Pick animals with red fluorescence to maintain zfEx416. Reference: Shipley FB, et al. Front Neural Circuits. 2014 Mar 24;8:28.
SK4005 zdIs5 I. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. mec-4::GFP is expressed in touch neurons.
TL24 zdIs5 I; clr-1(cy14) II; slt-1(eh15) X. C. elegans zdIs5 [mec-4p::GFP + lin-15(+)] I. cy14 was isolated in a screen for suppressors of the AVM axon ventral guidance defect of slt-1 null mutant. cy14 is a G-to-A transition in the splice acceptor of intron 5 of clr-1 that leads to the use of a cryptic splice acceptor and consequently to an 18 bp deletion in exon 6.