Caenorhabditis Genetics Center (CGC)

Transgene Information

NamemuEx248 View on WormBase
Description[pNL209(daf-16::GFP::daf-16cDNA) + podr-1::RFP]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
CF1700 daf-16(mu86) I; mes-1(bn7) X; muEx248. C. elegans muEx248 [(pNL209) daf-16::GFP::daf-16(cDNA) + podr-1::RFP]. Pick green (body) / red (head neurons) animals. Transmission efficiency ~50%. Can be grown at 20C with some sterility (30-50%). The higher the temperture, the greater the sterility.
CF2247 daf-16(mu86) I; glp-1(e2141) III; muEx248. C. elegans muEx248 [daf-16::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain.
CF2278 daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx248. C. elegans muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain.
CF2288 daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx248. C. elegans muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain.