Transgene Information

NamevizIs23 View on WormBase
Description[Ppie-1::gfp::daf-2; unc-119 (+)]
ReporterGFP

Strains carrying this transgene

Strain Genotype Species Description
AUM2023 daf-2(e1370) unc-119(ed3) III; vizIs23. C. elegans vizIs23 [pie-1p::GFP::daf-2(WT)::pie-1 3'UTR + unc-119(+)]. Maintain at 15C; pick superficially wild-type animals to avoid silencing of the transgene. pie-1 driven DAF-2 coding region with GFP transgene rescues the germline defects of daf-2(e1370). Slow growing. The transgene is sometimes silenced in the germline resulting in dauerunc animals at 25C. Reference: Lopez AL 3rd, et al. Dev Cell. 2013 Oct 28;27(2):227-40.