Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.

ZK1307.8. Homozygous. Outer Left Sequence: ACACAAAGAAATTGGGGCTG. Outer Right Sequence: AAATTCAAAGAGTTCCCGCC. Inner Left Sequence: ATATCGGCATGAGACCCATC. Inner Right Sequence: TCATTCAAGCGAAACAAGGA. Inner Primer PCR Length: 2131 bp. Deletion Size: 1446 bp. Deletion left flank: ACTGACACCTTCCGATGCTTGGACGGATCC. Deletion right flank: TGACAAGGAAGTTGTTCACGAGGAACTTTA. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZK1307.1 Homozygous. Outer Left Sequence: tcacctccttattggttgcc. Outer Right Sequence: atttgccggtgtcttttgag. Inner Left Sequence: tgcaggtgggtagtagaggg. Inner Right Sequence: agatttacaagggttcgggg. Inner Primer PCR Length: 1192. Deletion size: about 500 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZK1307.1. Homozygous. Outer Left Sequence: TCACCTCCTTATTGGTTGCC. Outer Right Sequence: ATTTGCCGGTGTCTTTTGAG. Inner Left Sequence: TGCAGGTGGGTAGTAGAGGG. Inner Right Sequence: AGATTTACAAGGGTTCGGGG. Inner Primer PCR Length: 1193 bp. Deletion Size: 626 bp. Deletion left flank: ATCTGGAAGACCCTCTGTTTGCTCCATTGT. Deletion right flank: AACAATGTACACCCGTGGTCATTTTATTAT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZK1307.6. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807