Deficient in synthesis of ether-linked lipids. Contains high content of saturated fatty acids. Reference: Shi X, et al. J Lipid Res. 2016 Feb;57(2):265-75.

This strain is tolerant to acute treatment of ethanol. The severity and incidence of stress-induced tremors are greater than in wild-type. Reference: Jee C, et al. Genes Brain Behav. 2013 Mar;12(2):250-62.

Dopamine receptor knockout.

mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Heterozygotes are WT with dim GFP signal in the pharynx. mIs11 homozygotes are WT with bright GFP in the pharynx. rad-51(lg8701) homozygotes are non-GFP and throw dead eggs.

mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Heterozygotes are wild-type with dim GFP signal in the pharynx. mIs11 homozygotes are wild-type with bright GFP in the pharynx. ieDf2 homozygotes (non-GFP) develop normally but produce 97.5% inviable embryos and a high frequency of males among the surviving self-progeny. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. ieDf2 is a deficiency of zim-1, zim-2, zim-3, and him-8 generated by MosDel, resulting in single-copy insertion of a copy of the C. briggsae unc-119 gene on Chromosome IV. The deletion spans the sequences from the beginning of the zim-1 coding sequence through the ttTi22866 Mos1 insertion site.

Reduced gut fluorescence, dull green. M-MATING+++ 10-30%WT.

Increased gut fluorescence, blue. M-MATING++++ >30%WT.

Unc.

Unc. Weak coiler.

Chemotaxis abnormal. Amphid defect-EM. M-MATING-NO SUCCESS

Unc. DNAse undetectable. Gut DNA fluoresence abnormal. M-MATING-NO SUCCESS.

Dauer defective. Notched head. Unlinked double. M-MATING-NO SUCCESS.

Catecholamine abnormal. Recessive. M-MATING++ 1-10%WT. Previously called ctl-2.

Temperature sensitive. At 15C, L1 larvae are coilers, adults are wild-type. At 25C, severe kinker, some coiling.

Migration defective: pre VC. Variable expressivity. Reverse kinker as adult. L1 moves well. Temperature sensitive. Can be maintained at 20C.

Slow moving Unc. Body Muscle abnormal. M-MATING-NO SUCCESS.

6% XO self-progeny. Males sterile. M-MATING-NO SUCCESS.

Male gonad abnormal. Males sterile. 6% XO self-progeny. M-MATING-NO SUCCESS.

Dpy. Temperature sensitive.

Medium Dpy.

Vulvaless. M-MATING++ 1-10%WT.

Egg-laying defective. Moderate to severe bloating. 30% make bags of worms. Males mate. [NOTE: Probably contains a tightly linked mutation which cannot be crossed off. Use MT3188 as reference strain. Michael Stern 6\97]

Dpy. Roller left hand. Temperature sensitive.

Mechanosensory abnormal. Scored with difficulty. Sometimes sensitive in the tail. M-MATING++ 1-10%WT.

Mechanorsensory abnormal. Touch insensitive. Lethargic. M-MATING+++ 10-30%WT.

Dauer defective. Non-crowder. M-MATING++++ >30%WT.

Dauer defective. Crowds. Leaky. Dauer recovery normal. Chemotaxis defective-Na+. M-MATING++++ >30%WT.

Unc.

DNAse undetectable. M-MATING++++ >30%WT.

Dpy.

Marginally Unc-kinky backing. Temperature sensitive. Migration pre VC defect. M-MATING+POOR <1%WT. Can be maintained at 20C.

Mechanosensory abnormal - semidominant. Lethargic at 25C. Neurons/Touch abnormal.

15% XO self-progeny. 6% XXX self-progeny. Low brood size. 78% unhatched eggs. General non-disjunction. Recessive.

37% XO self-progeny. 6% XXX self-progeny. Recessive. M-MATING++ 1-10%WT. [Background mutation that causes rays 8 and 9 to fuse.]

Mechanosensory abnormal. Temperature sensitive. Mec at 25C. WT at 15C.

Unc.

Touch insensitive. Lethargic.

XX animals are WT. XO animals are fertile hermaphrodites.

Unc.

Coiler Unc. Dominant.

Transforms XX to males. Variable expression. Masculinized self-fertile hermaphrodite. tra-2(f70) was crossed into the N2 background to construct a dpy-10 tra-2 unc-4 triple mutant, then crossed to remove the flanking dpy and unc (Jonathan Hodgkin).

Mechanosensory abnormal. Touch insensitive. Dominant. Cells become vacuolated and die.

Dpy. Recessive. M-MATING+POOR <1%WT.

Suppressed Unc. Movement almost WT.

Dauer defective. Long.

Dauer defective. Long.

Long. Mechanosensory abnormal. Semidominant Mec.

Lon. Unc.

Severe Unc-paralyzed. Growth good. Lannate resistant.

Maternal effect tra-3. Heterozygotes are WT and segregate WT and Dpy. Homozygous tra-3 animals are WT hermaphrodites which segregate abnormal sterile males or intersex.