mScarlet inserted at the C-terminus of the endogenous col-63 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing the mNeonGreen cassette in syb5014. Reference: Ragle JM, et al. bioRxiv 2025.05.14.653803; doi: https://doi.org/10.1101/2025.05.14.653803.