Caenorhabditis Genetics Center (CGC)

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Strain Species Genotype
JDW652 C. elegans rol-8(wrd230[rol-8mNG::3xFLAG]) II. Show Description
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous rol-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs.