| JH3148 |
C. elegans |
ltIs37 IV; meg-1(vr10) X; axIs1522. Show Description
axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Wang JT, et al. eLife 2014;3:e04591. |
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| JH3150 |
C. elegans |
ltIs37 IV; meg-1(vr10) meg-3(tm4259) X; axIs1522. Show Description
axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. |
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| JH3156 |
C. elegans |
ltIs37 IV; pptr-1(tm3103) V; meg-1(vr10) X; axIs1522. Show Description
axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. |
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| JH3229 |
C. elegans |
meg-1(vr10) meg-3(tm4259) X. Show Description
P granule defect. Some sterility. Reference: Wang JT, et al. eLife 2014;3:e04591. |
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| JH4877 |
C. elegans |
nos-2(egx211[7xHA::nos-2]) II; meg-1(vr10) X. Show Description
7xHA tag inserted at N-terminus of endogenous nos-2 locus. Minor maternal effect sterility at 20C; increased sterility at 25C. Derived by crossing parental strains YL139 and JH4876. NOTE: egx211 was originally designed as 8xHA tag but sequencing confirmed a point mutation disrupts one repeat, making it a functional 7xHA tag. egx211 CRISPR Guide sequence: TTCTTCTTGAAAGATGTCTC. Reference: Simmons WR, et al. 2026 bioRxiv 2026.07.01.735846; doi: https://doi.org/10.64898/2026.07.01.735846. |
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| YL139 |
C. elegans |
meg-1(vr10) X. Show Description
Maternal effect sterility at 25 degrees. Can be maintained at 20C. Deletion breakpoints: AGATGCCACATACAAACGCT / CTGGCGGAAGACGATGCAAA Reference: Leacock SW & Reinke V. Genetics. 2008 Jan;178(1):295-306. |
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