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F59D8.2 Homozygous. Outer Left Sequence: cagcgtgagcattttgagaa. Outer Right Sequence: caaagctgaggtcaacccat. Inner Left Sequence: cgaagacggtttcgaatgat. Inner Right Sequence: tcaaggctatcgagatagagca. Inner Primer PCR Length: 1165. Deletion size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

zcIs4 [hsp-4::GFP] V. vit-4(ac4) is a dominant allele that causes ER stress. Since vit-4 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17.

Multiple mutations were simultaneously introduced into the vit-3, vit-4, and vit-5 loci using a single crRNA to produce vit-5(rhd322[T391E, L392F, A393*]), vit-4(rhd321[T391E, L392F, A393*]), and vit-3(rhd320[T391E, L392F, A393*]). A similar strategy was used to create vit-6(rhd332[S320F, W322*]). vit-2(rhd352) is a CRISPR-engineered deletion removing the entire vit-2 coding sequence generated in parental strain DLS1004 to produce DLS1102. Reference: Macharios MM, et al. MicroPubl Biol. 2025 Sep 10;2025:10.17912/micropub.biology.001789. doi: 10.17912/micropub.biology.001789. PMID: 41018027.