Search Strains

F59D8.1 Homozygous. Outer Left Sequence: atgcggtcgttctcaacttc. Outer Right Sequence: gcacattgctgaccttctca. Inner Left Sequence: cggtggaattcctcaacatc. Inner Right Sequence: ggatttgcttcaaagaccca. Inner Primer PCR Length: 3061. Deletion size: about 1800 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Multiple mutations were simultaneously introduced into the vit-3, vit-4, and vit-5 loci using a single crRNA to produce vit-5(rhd322[T391E, L392F, A393*]), vit-4(rhd321[T391E, L392F, A393*]), and vit-3(rhd320[T391E, L392F, A393*]). A similar strategy was used to create vit-6(rhd332[S320F, W322*]). vit-2(rhd352) is a CRISPR-engineered deletion removing the entire vit-2 coding sequence generated in parental strain DLS1004 to produce DLS1102. Reference: Macharios MM, et al. MicroPubl Biol. 2025 Sep 10;2025:10.17912/micropub.biology.001789. doi: 10.17912/micropub.biology.001789. PMID: 41018027.

mCherry knocked into C terminal of vit-3 by CRISPR/Cas9 in the background of parental strain BCN9071 vit-2(crg9070[vit-2::gfp]) X. This resulting double-labelled strain was crossed six times with N2 to remove potential off-target mutations. mCherry and GFP are co-localized in the intestine, body cavity, oocyte, and embryo in adult hermaphrodites. Reference: Zhai C, et al. Aging cell, 21(11), e13719. https://doi.org/10.1111/acel.13719 PMID: 36199214.

rhdSi42 [vit-3p::mCherry::unc-54 3'UTR + Cbr-unc-119(+)] II. Red fluorescence in the intestines of adults. Reference: Cully D, et al. MicroPubl Biol. 2024 Sep 30;2024:10.17912/micropub.biology.001163. doi: 10.17912/micropub.biology.001163. PMID: 39410967.