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Strain Species Genotype
MT3286 C. elegans unc-93(e1500n1412) III. Show Description
Wild type. n1412 loss-of-function suppresses the e1500sd rubberband phenotype.
MT3289 C. elegans unc-93(e1500n1415) III. Show Description
Wild type. n1415 loss-of-function suppresses the e1500sd rubberband phenotype.
MT3329 C. elegans unc-93(e1500n1419) III. Show Description
Wild Type.
MT3661 C. elegans sup-9(n1550) II; unc-93(n1415) dpy-17(e164) III. Show Description
Dpy. unc-93(n1415) loss-of-function suppresses the sup-9(n1550) Rubberband phenotype.
MT3840 C. elegans unc-93(e1500n1415) dpy-17(e164) III; twk-18(e1913)/+ X. Show Description
Pick DpyUnc worms to maintain, as e1913 is a dominant Unc and a recessive lethal. Actually, the Uncs are not very Dpy, so pick non-Dpy-looking animals that don't move well. e1913 previously called unc-110.
MT4535 C. elegans unc-93(e1500n1912) III. Show Description
Non-Unc.
MT534 C. elegans unc-93(e1500) dpy-17(e164) III. Show Description
DpyUnc. Semi-dominant Unc.
MT573 C. elegans dpy-1(e1) unc-93(e1500) III. Show Description
DpyUnc. e1500 is semi-dominant.
MT5896 C. elegans sup-9(n1550) II; unc-93(n2289) sup-18(n1014) III. Show Description
MT5920 C. elegans nP48 sup-9(n1330) II; unc-93(e1500) III. Show Description
Non-Unc phenotype.
MT6015 C. elegans unc-93(n2334) dpy-17(e164) III; sup-10(n983) X. Show Description
n2334 suppresses n983. Dpy.
MT690 C. elegans nDf6/unc-93(e1500) dpy-17(e164) III. Show Description
Heterozygotes are Unc (Rubberband) and segregate Unc, DpyUnc and early larval lethals (nDf6 homozygotes). Maintain by picking Unc non-Dpy.
MT691 C. elegans nDf7/unc-93(e1500) dpy-17(e164) III. Show Description
Hets are Unc(Rubberband) and Egl. Segregate Unc, DpyUnc and early larval lethals (L1). Maintain by picking Uncs. Received new stock 1/3/94.
MT692 C. elegans nDf8/unc-93(e1500) dpy-17(e164) III. Show Description
Hets are Unc(Rubberband) and Egl. Segregate Unc, DpyUnc, and dead eggs. Maintain by picking Uncs.
MT696 C. elegans nDf12/unc-93(e1500) dpy-17(e164) III. Show Description
Hets are Unc (Rubberband) and Egl. Segregate Unc, DpyUnc and dead eggs. Maintain by picking Uncs. CGC received new stock 8/98.
MT697 C. elegans nDf13/unc-93(e1500) dpy-17(e164) III. Show Description
Heterozygotes are Unc(Rubberband) and Egl, and segregate Unc, DpyUnc and dead eggs. Maintain by picking Unc.
MT699 C. elegans nDf15/unc-93(e1500) dpy-17(e164) III. Show Description
Heterozygotes are Unc and segregate Unc, DpyUnc and dead eggs. Maintain by picking Unc.
MT725 C. elegans unc-93(e1500) III; sup-10(n183) X. Show Description
Non-Unc.
MT765 C. elegans unc-93(e1500n224) III. Show Description
WT phenotype.
MT775 C. elegans unc-93(e1500n234) III. Show Description
Wild type phenotype. e1500 rubberband phenotype is suppressed by the loss-of-function n234 mutation.
MT912 C. elegans sup-11(n403) I. Show Description
Dominant suppressor of unc-93(e1500), recessive small scrawny slow growing phenotype.
OH122 C. elegans mgIs25. Show Description
mgIs25 [unc-97::GFP]. Translational fusion of the complete encoding sequence of unc-97.
OH4460 C. elegans otEx2572. Show Description
otEx2572 [unc-97::NLS::GFP]. GFP expressed strongly in muscle. Maintain by picking GFP+ animals.
OH4461 C. elegans lin-15B&lin-15A(n765); otEx2571. Show Description
otEx2571 [unc-97::GFP + lin-15(+)]. GFP strongly expressed in muscle. Maintain by picking GFP+, non-Muv animals.
RW1029 C. elegans unc-54(e1301) I; unc-90(e1463) X. Show Description
Lethal. Cold sensitive. Class 3 allele. Poor viability.
SP1784 C. elegans dpy-1(e1) ncl-1(e1865) unc-36(e251) III; him-8(e1489) IV; mnDp90 [dpy-1(+) ncl-1(+) unc-36(+) unc-93(+)] (III;f). Show Description
Animals with mnDp90 are WT. Animals which have lost mnDp90 are DpyUnc and Ncl. Throws males. Males containing mnDp90 are fertile. mnDp90 was derived from mnDp86.
SP457 C. elegans unc-93(e1500) III. Show Description
Rubberband Unc. Reverts. There is some question regarding e1500 in this strain. Please see MT19321 as a better strain.
TY1353 C. elegans yDf10 unc-32(e189)/unc-93(e1500) dpy-17(e164) III. Show Description
Heterozygotes are Unc-93 and segregate more Unc-93, yDf10 homozygotes (dead eggs) and Unc-93 Dpy-17 homozygotes (young dpy-17 larvae are easily recognizable as abnormal spindle-shaped things). Difficult to maintain and use. yDf10 apparently causes semi-sterility (a second strain constructed by the Mark Edgley at the CGC, yDf10/qC1, exhibits same sterility), and unc-93 is Egl and difficult to mate into. Some Df homozygotes are laid, but most remain inside the mother.
VC2228 C. elegans +/szT1 [lon-2(e678)] I; unc-97(ok2760)/szT1 X. Show Description
F14D12.2. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok2760 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: GTGGCCAACTTTCAGTGGTT. External right primer: TGCGCTTTTTCAATTCTGTG. Internal left primer: CGACCACAACCATATCAACG. Internal right primer: CGTTTGCATGTTGGTTTCAT. Internal WT amplicon: 1239 bp. Deletion size: 516 bp. Deletion left flank: GGATGTTTCTGTTGTGAGATTTGCAATAAA. Deletion right flank: AACAGCACTTCCACAAGGTACTTGAAATAT. Insertion Sequence: AAGATTTGCAAAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC627 C. elegans unc-95(ok893) I. Show Description
Y105E8A.6. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC828 C. elegans unc-94(ok1210) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
C06A5.7a. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok1210 homozygotes (grotty sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
ZW127 C. elegans zwIs106. Show Description
zwIs106 contains [unc-9p::GFP + lin-15(+)]. Superficially wild-type. Maintain under normal conditions. Described in Altun et al. Dev Dyn 238:1936-50 (2009).