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Strain Species Genotype
CB1951 C. elegans unc-42(e270) sma-1(e30) V. Show Description
Small. Unc. Linked closely.
CB2222 C. elegans unc-42(e270) unc-41(e268) V. Show Description
Growth slow. Severe Unc. Linked closely.
CB2261 C. elegans dpy-5(e61) unc-40(e1430) I. Show Description
DpyUnc.
CB268 C. elegans unc-41(e268) V. Show Description
Unc. M-MATING-NO SUCCESS.
CB270 C. elegans unc-42(e270) V. Show Description
Unc. Recessive. Mapping marker standard. M-MATING+POOR <1%WT.
CB271 C. elegans unc-40(e271) I. Show Description
Unc. Small. Egg Retainer. Serotonin Def. Recessive. M-MATING-NO SUCCESS.
CB2810 C. elegans tra-1(e1575)/+ III; unc-42(e270) him-5(e1490) dpy-21(e428) V. Show Description
Pick Unc hermaphrodite to maintain (these will be tra-1/+; unc-42 him-5 dpy-21 XO animals). Segregates Unc hermaphrodites (tra-1/+ III), Unc males (+/+ III), and DpyUnc hermaphrodites (+/+ III). dpy-21 not expressed in XO. dpy-21(e428) V; XX animals are weak Dpy. dpy-21(e428) V; XO animals are non-Dpy.
CB286 C. elegans unc-45(e286) III. Show Description
Slow moving Unc. Body muscle abnormal. Temperature sensitive. Recessive. M-MATING+POOR <1%WT.
CB307 C. elegans unc-47(e307) III. Show Description
Unc. Recessive. M-MATING++ 1-10%WT.
CB362 C. elegans unc-44(e362) IV. Show Description
Paralyzed Unc. Small. Recessive. M-MATING-NO SUCCESS.
CB382 C. elegans unc-49(e382) III. Show Description
Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small. M-MATING++ 1-10%WT
CB4035 C. elegans fem-2(e2105)/unc-45(r450) dpy-1(e1) III. Show Description
Heterozygotes are WT and segregate WT and DpyUnc. 1/3 of the WT are fem-2 homozygotes. Homozygous fem-2 animals are hermaphrodites if mother was heterozygous for fem-2, and are fertile females if mother was homozygous for fem-2.
CB407 C. elegans unc-49(e407) III. Show Description
Uncoordinated, shrinker.
CB408 C. elegans unc-43(e408) IV. Show Description
Marginally Unc-no sin wave. Lethargic. Recessive. M-MATING-NO SUCCESS.
CB419 C. elegans unc-42(e419) V. Show Description
Unc. Recessive.
CB5378 C. elegans unc-42(e270) let-560(e2658) V/nT1 (IV;V). Show Description
let-560(e2658) was a spontaneous mutation in strain DH424. let-560 homozygotes are late embryonic lethal. Strain segregates WT, Vul and dead eggs.
CB623 C. elegans unc-42(e623) V. Show Description
Unc.
CG118 C. elegans unc-43(sy574) IV; him-5(e1490) V. Show Description
Males constitutively protract their spicules in the absence of mating cues.
CG460 C. elegans unc-43(sy574) IV; him-5(e1490) V; rgEx161. Show Description
rgEx161[lev-11p::UNC-43g + gtl-1p::CFP]. rgEx161 rescues unc-43(sy574)-induced spicule protraction.
CGC138 C. elegans unc-13(e51)/hT1 I; dpy-11(e224)/hT1 [unc-42(e270) umnIs79] V. Show Description
umnIs79 [myo-2p::GFP + NeoR, I: 6284001] I. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc, arrested hT1 homozygotes (GFP+), and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9.
CGC139 C. elegans unc-13(e51)/hT1 I; dpy-11(e224)/hT1 [unc-42(e270) umnIs80] V. Show Description
umnIs80 [myo-2p::mKate2 + NeoR, I: 6284001] I. Pick wild-type mKate2+ to maintain. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, arrested hT1 homozygotes (mKate2+), and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9.
CGC20 C. elegans dpy-18(e364)/eT1 III; unc-46(e177)/eT1[umnIs9] V. Show Description
umnIs9 [myo-2p::GFP + NeoR, III:9421936 (intergenic)] V. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-36 GFP+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC23 C. elegans dpy-18(e364)/eT1 [umnIs12] III; unc-46(e177)/eT1 V. Show Description
umnIs12 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] III. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-36 GFP+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC29 C. elegans unc-13(e51)/hT1 [umnIs18] I; dpy-11(e224)/hT1 [unc-42(e270)] V. Show Description
umnIs18 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Dpy Unc, arrested hT1 homozygotes, and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9.
CGC60 C. elegans dpy-18(e364)/eT1 III; unc-46(e177)/eT1[umnIs46] V. Show Description
umnIs46 [myo-2p::mKate2 + NeoR, III:9421936 (intergenic)] V. Heterozygotes are wild-type mKate+, and segregate wild-type mKate2+, Unc-36 mKate+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC69 C. elegans dpy-18(e364)/eT1 [umnIs55] III; unc-46(e177)/eT1 V. Show Description
umnIs55 [myo-2p::mKate2 + NeoR, V:1005689 (intergenic)] III. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, Unc-36 mKate+(eT1), dead eggs, and DpyUncs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9.
CGC75 C. elegans unc-13(e51)/hT1 [umnIs58] I; dpy-11(e224)/hT1 [unc-42(e270)] V. Show Description
umnIs58 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] V. Pick wild-type mKate2+ to maintain. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, arrested hT1 homozygotes (mKate2+), and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9.
CX652 C. elegans kyIs235 V; syg-1(ky652) X. Show Description
kyIs235 [unc-86::snb-1::YFP + unc-4p::lin-10::RFP(intron) + odr-1::RFP]. Also known as unc-86::VAMP::YFP. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
CZ24990 C. elegans unc-44(ju1412[unc-44::GFP]) IV. Show Description
Endogenous unc-44 locus tagged for UNC-44L::GFP expression. The long isoform of UNC-44 is specific to neuronal expression. Reference: Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707.
CZ25013 C. elegans unc-44(ju1413[unc-44::gfp::LoxP::3xflag]) IV. Show Description
unc-44(ju1413[unc-44::GFP::LoxP::3xflag]) IV. UNC-44C (short isoform of UNC-44) tagged with GFP. UNC-44C is strongly expressed in multiple tissues: nervous system (from 1.5-fold stage to adult), epidermis (from early embryo to adult), seam cells (from L1 to L4), vulva (from L3 to adult), and spermatheca/sheath cells (from L4 to adult). Reference: Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707.
DA443 C. elegans rol-3(e754) unc-42(e270) V. Show Description
Roller Unc. e754 is cold-sensitive: lethal at 15C.
DA675 C. elegans unc-45(r450) dpy-1(e1) phm-3(ad493) III. Show Description
Dpy. Unc (ts). Phm. Weak pharyngeal birefringence.
DG1856 C. elegans goa-1(sa734) I. Show Description
Recessive, early stop mutation within the coding sequence (C to T substitution in aa52) makes sa734 a likely null allele. May grow slightly better at 15C. Hyperactive, lays early stage eggs, increased amplitude of locomotort wave-form. Suppresses the lethargy and egg-laying defects of unc-43(n498). Reverses direction of locomotion more frequently than WT.
DG832 C. elegans emb-30(tn475)/eT1 III; unc-46(e177) mdf-1(gk2)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-46 Steriles, and Unc-36 (eT1 homozygotes). Maintain by picking WT.
DG844 C. elegans emb-30(tn377) III; unc-46(e177) mdf-1(gk2) V. Show Description
emb-30(tn377ts) conditionally suppresses mdf-1(gk2). Maintain at 15C.
DL7 C. elegans wdIs4 II; unc-3(n3435) X. Show Description
wdIs4 [unc-4p::GFP + dpy-20(+)] II.
DP246 C. elegans unc-45(st601)/sC1 [dpy-1(s2170)] III. Show Description
Made from LV15. st601 is a non-conditional two-fold arrest lethal.
DR1056 C. elegans unc-42(e270) ama-2(m323) V/nT1 [let-?(m435)] (IV;V). Show Description
Heterozygotes are WT and segregate WT and dead eggs. (Chromosome carrying unc-42 ama-2 is homozygous lethal.) Strain is resistant to _-amanitin.
DR108 C. elegans dpy-11(e224) unc-42(e270) V. Show Description
DpyUnc.
DR1213 C. elegans unc-44(e362) unc-24(e138) IV. Show Description
Unc-44 masks the Unc-24 phenotype. Paralyzed coiler, Dpy, tends to curl.
DR1228 C. elegans unc-45(e286) daf-7(e1372) dpy-1(e1) III. Show Description
Dpy. Temperature sensitive dauer constitutive (leaky). Temperature sensitive Unc. At 15C, Dpy adults and some dauers. At 25C, DpyUnc dauers and some DpyUnc adults (leakiness varies: 60-95% dauers).
DR1799 C. elegans daf-7(n696)/unc-45(st604) III. Show Description
WT phenotype. Segregates WT, constitutive dauers and ts mild Uncs. Uncs are maternal-effect lethal: F1 Uncs from a heterozygote produce only arrested 2-fold stage progeny that hatch. Grow at 22.5C (n696 produces nearly 100% dauers at this temperature).
DR240 C. elegans dpy-11(e224) unc-42(e270) daf-11(m84) V. Show Description
Temperature sensitive dauer constitutive. Dpy. Unc.
DR789 C. elegans dpy-13(e184) IV/nT1 [let-?(m435)] (IV;V); unc-42(e270) V/nT1 [let-?(m435)] (IV;V). Show Description
Heterozygotes are WT and segregate WT, DpyUnc and dead eggs. Maintain by picking WT.
DR94 C. elegans unc-45(m94) III. Show Description
Temperature sensitive Unc. Slow at 15C. Paralyzed at 20C and 25C.
EA90 C. elegans pag-1(ls2) dpy-17(e164) III. Show Description
Dpy. pag-1(ls2) is recessive and has not visible phenotype by itself. It increases the expression of certain lacZ fusion genes such as lacZmec-7, unc-86lacZ and unc-4unc-76lacZ.
EAK102 C. elegans eeeIs1. Show Description
eeeIs1 [unc-45::Htt513(Q15)::YFP::unc-45 3'UTR]. YFP expression in body wall muscle cells. YFP is fused to a fragment of mutant human Huntingtin protein. Reference: Lee AL. et al. PLoS One. 2017 Mar 10;12(3):e0173644.
EAK103 C. elegans eeeIs2. Show Description
eeeIs2 [unc-45::Htt513(Q128)::YFP::unc-45 3'UTR]. Motility defect. YFP expression in body wall muscle cells. YFP is fused to a fragment of mutant human Huntingtin protein. Reference: Lee AL. et al. PLoS One. 2017 Mar 10;12(3):e0173644.
EG1285 C. elegans lin-15B&lin-15A(n765) oxIs12 X. Show Description
oxIs12 [unc-47p::GFP + lin-15(+)]. Integration maps at +2.0 on X. Expression of GFP in all GABAergic neurons.
EG1653 C. elegans oxIs22 II. Show Description
oxIs22 [unc-49p::unc-49::GFP + lin-15(+)]. Psoralen integration of oxEx129 [unc-49Bp(long)::GFP + lin-15(+)]. Dorsal and ventral.