Animals with the duplication are Unc.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and are embyronic lethals.

Maintain by picking Uncs. DpyUncs arrest as early larvae. s2582 previously thought to be an allele of let-726.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in the early larval stage.

Maintain by picking Uncs. DpyUncs arrest as early larvae.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage.

Maintain by picking Uncs. DpyUncs become sterile adults.

Maintain by picking Uncs. DpyUncs arrest at early-mid larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as late larvae or sterile adults.

Maintain by picking Uncs. DpyUncs arrest at early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and are probably embyronic lethals.

Maintain by picking Uncs. DpyUncs become sterile adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in late larval development.

Maintain by picking Uncs. DpyUncs arrest at early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and become sterile adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc are adult steriles.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval stages.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs which arrest as early larvae.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs which arrest as early larvae.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage.

Maintain by picking Uncs. DpyUncs become sterile adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as sterile adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults. Note: let-733(s2621) seems to interact with let-749(s2467).

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid larval development.

Maintain by picking Uncs. After 3 days at 20C the DpyUncs are at the mid-larvae stage; by day 10 they are adults giving some eggs which hatch sick looking larvae.

Maintain by picking Uncs. DpyUncs arrest at mid-larval stage. s2630 pka let-745.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage. s2631 previously thought to be an allele of let-731.

Maintain by picking Unc-32 animals.

Maintain by picking Unc-32 animals.

Maintain by picking Unc-32 animals. DpyUncs arrest development at early larval stages.

Maintain by picking Unc-32 animals.

Maintain by picking Uncs.

Maintain by picking Uncs. DpyUncs arrest at early-mid larval stages.

Maintain by picking Unc-32 animals. Dpy animals that have lost sDp3 should also be Mel; expected Mel phenotype is embryonic lethal.

Heterozygotes are Muv and Egl (n137 is semi-dominant) and segregate DpyMuv and Unc lethals (most arrest as L1-L3, the few survivors are sterile, scrawny, Unc and have a large ventral blip at hte position of the vulva). Maintain by picking non-Dpy non-Unc.

syIs20 [gpa-1::lacZ + dpy-20(+)] V. Unc. Him. p-Vul. Abnormal secondary vulva lineage defect in 10% of animals. Reduced brood size as well as other pleiotropic defects. Males have ray defect (cannot mate) and SPD sheath to neuron transformation, which can be visualized by lacZ staining in syIs20 [gpa-1::lacZ] background: 1 cell per spicule stains in WT (the SPD neuron) whereas in hmg-1.2(sy549), 2 cells per spicule stain. sy549 previously called son-1. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Animals with the duplication are WT. Animals which have lost the duplication are DpyUnc-36.

ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, SmaUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2.

ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2.

ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2.

ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest as embyros or in larval development. Not well balanced. Neurons visualized with ncIs2.

ccEx7271 [let-858::GFP + pha-1(+)]. This strain expresses nuclear-localized GFP in all somatic nuclei, but reduced or no GFP in germ cells. If maintained at 20C, pha-1(ts) genotype will select for transgenic animals. Germ cell expression can be observed when maintained at 25C. Germline transgene silencing is abnormal.

Heterozygotes are Unc-93 and segregate more Unc-93, yDf10 homozygotes (dead eggs) and Unc-93 Dpy-17 homozygotes (young dpy-17 larvae are easily recognizable as abnormal spindle-shaped things). Difficult to maintain and use. yDf10 apparently causes semi-sterility (a second strain constructed by the Mark Edgley at the CGC, yDf10/qC1, exhibits same sterility), and unc-93 is Egl and difficult to mate into. Some Df homozygotes are laid, but most remain inside the mother.

Unc. Throws Unc, UncLon males and dead eggs.

Unc. Throws Unc, UncLon males and dead eggs.

Unc. Throws Unc, UncLon males and dead eggs.

Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Heterozygotes are WT and segregate WT, DpyUncs and Unc Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.