Coiler Unc. Severe. Recessive. M-MATING-NO SUCCESS.

Extreme coiler from L1 to adult. Hypomorph. f131/nDf17 is L1 lethal. Molecular lesion: g to a transition in splice donor site of exon 6 (g3485a) in ZK637.8 gene encoding a V-ATPase alpha subunit.

Heterozygotes are WT and segregate WT heterozygote, Uncs (unc-32 homozygotes), and Steriles (dcr-1 homozygotes).

Temperature sensitive. Maintain at 15 degrees. Germline tumor formation at 25 degrees. Proximal proliferations defects. Reference: Pepper et al. (2003) Genetics 163(1):115-32.

Long. Unc. Linked closely.

Dpy. Coiler Unc. Closely linked. Doesn't grow at 25C.

Temperature sensitive dauer constitutive. Unc. Closely linked.

Unc-32 hermaphrodites segregating Unc hermaphrodites and Unc Tra homozygous XX pseudomales with variable male tails, hermaphroditic gonads and adult yolk production. Class B2 allele (male tail, sterile hermaphrodite gonad) derived by mutation of tra-(gf) allele e1575. Reference: Hodgkin (1987) PMID:3428597.

Unc. Temperature sensitive. Maintain at 15C. hc70 is semi-dominant.

Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency).

Temperature senstive dauer constitutive. Unc-coiler. 100% dauers at 25C.

Unc-coiler. Segregates males. M-MATING-NO SUCCESS.

Heterozygotes are WT and segregate WT, dauer constitutive, and Uncs. Lethal dauers.

Homozygous viable and fertile, but slow growing, transparent and small. Originally from BC4253 but outcrossed an additional 7 times.

Unc. Temperature sensitive tim-1. Viable at 15C. Mel at 25C.

Dpy Unc.

Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Unc. ku1 pka sur-1(ku1).

Egl. Unc.

Unc.

Unc. Persistent cell corpses-Maternal effect.

Maintain by picking wild-type animals raised at 25C. Heterozygotes will be wild-type and segregate wild-type, Unc, and Sterile Muv. The phenotype of homozygous lin-13 hermaphrodites segregating from a heterozygous mother depends on the temperature at which the strain was grown. At 25C, homozygous hermaphrodites segregating from a heterozygote are both Muv and sterile. At 20C, ~1/2 of hermaphrodites segregating from a heterozygote are sterile, but only a few are Muv. At 15C, hermaphrodites segregating from a heterozygote are almost wild type in appearance and fertility. However, if the progeny of these 15C animals are grown at 15C, all are sterile and some are Muv. If the progeny of these 15C animals are grown at 25C, then some animals arrest during larval growth and the rest are both sterile and Muv. Reference: Ferguson EL & Horvitz HR. Genetics. 1985 May;110(1):17-72. PMID: 3996896.

DpyUnc.

RNAi deficient. Unc.

Unc. Animals which have lost the duplications are Sterile and Unc.

Maintain by picking Unc-32 animals.

Unc strain. Aged: Original sDf121 strain (BC4201) was maintained on plates for approx. 2 years, and then frozen as BC4697. See also WBPaper00003827.

Heterozygotes are WT and segregate WT, Uncs which are sterile and dead eggs (eDf2 homozygotes). Strain breaks down very rarely.

Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. The DpyUncs will outgrow the hets.

Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.

Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.

Heterozygotes are WT and segregate WT, Unc-32 maternal effect lethals (Mel) and Unc-36.

Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.

syIs80 [(pPGF11.13) lin-11::GFP + unc-119(+)] III. Animals are Unc. GFP fluorescence is observed in vulval cells, uterine pi cells and VC neurons.

Heterozygotes are Dpy and segregate Dpy, DpyUnc and dead eggs.

Unc. Temperature sensitive hypomorphic lin-12 allele. At restrictive temperature of 25C: 100% Egl in unc-32 background (95% Egl without unc-32); 30% have 2 anchor cells, some proximal mitosis, abnormal vulval lineages but has opening; leaks some eggs; mates with males. At permissive temperature of 15C: 93% Egl(+) and 7% Egl(-) [lin-12(d)-like: no anchor cell and no vulval opening]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Heterozygotes are WT and segregate WT, Sterile Unc coilers, Unc-36s (eT1 homozygotes) and dead eggs. Pick WT to maintain. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Heterozygotes are Dpy and segregate Dpy, DpyUncs and dead eggs.

Heterozygotes are Dpy and segregate Dpy, DpyUnc and dead eggs. e1259 has a ts maternal effect.

Heterozygotes are WT and segregate WT, SmaUnc and dead eggs. Maintain by picking WT.

Animals with the extrachromosomal array are Unc Rollers. Animals which have lost the array are Pat. Strain can be propagated by chunking. The exact construct used to create stEx10 is unknown; it is thought to carry a pat-2 rescuing construct (most likely a cosmid) with a Rol marker.

WT strain which segregates WT, Unc-36, DpyUnc and dead eggs. DpyUncs give 6-10% viable progeny at 20C and less than 1% at 15C. Maintain by picking WT.

Temperature sensitive dauer constitutive. Long. Unc. Linked closely.

Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36.

Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-2 complementation.

Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-7 complementation.

Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-3.

Heterozygotes are WT and segregate WT, Uncs, and Lags (ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.