Caenorhabditis Genetics Center (CGC)

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Strain Species Genotype
GG36 C. elegans tyms-1(g36) I. Show Description
Temperature sensitive. Maintain at 15C. Will grow at 20C, but not 25C.
MJ65 C. elegans tyms-1(hc65) I. Show Description
ts embryonic lethal. Grows at 15C, 20C. Lethal at 25C.
AG247 C. elegans tyms-1(tm2429) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP tm2429 homozygotes (embryonic lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Reference: Jaramillo-Lambert A, et al. G3 (2015).