| AH3437 |
C. elegans |
tln-1(zh117[gfp::tln-1]) I. Show Description
Wild-type morphology. Endogenous GFP reporter for tln-1. Reference: Walser M, et al. PLoS Genet. 2017 Jan 30;13(1):e1006592.
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| DR49 |
C. elegans |
tln-1(e259) I. Show Description
Unc. Weakly semi-dominant. Scored with difficulty.
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| RB1445 |
C. elegans |
Y71G12B.11(ok1648) I. Show Description
Y71G12B.11 Homozygous. Outer Left Sequence: tgaagtggtggctcttgttg. Outer Right Sequence: aagttccgtttgttggttgc. Inner Left Sequence: tggtttctaaggggttgcag. Inner Right Sequence: gcgcttctctcaatttgtcc. Inner Primer PCR Length: 3151. Estimated Deletion Size: about 1100 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
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| DA1075 |
C. elegans |
tln-1(e259) dpy-5(e61) I. Show Description
Dpy. Unc.
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| OCF108 |
C. elegans |
unc-119(ed3) III; atln-1(ocf102[atln-1::GFP::3xFLAG]) IV; ocfIs2. Show Description
ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP::3xFlag tag inserted at C-terminus of endogenous atln-1 locus. ocf102 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| OCF116 |
C. elegans |
unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ojIs23. Show Description
ojIs23 [pie-1p::GFP::spcs-1 + unc-119(+)]. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| OCF162 |
C. elegans |
ebp-2(or1954[ebp-2::mKate2]) II; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mKate2 tag inserted into C-terminus of endogenous ebp2 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| OCF164 |
C. elegans |
spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous spd-5 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| OCF165 |
C. elegans |
spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ocfIs2. Show Description
ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous spd-5 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| OCF167 |
C. elegans |
tac-1(or1955[GFP::tac-1]) II; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous tac-1 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| OCF172 |
C. elegans |
unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ltIs78; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ltIs78 [pie-1p::GFP::TEV::Stag::air-1 spliced coding + unc-119(+)]. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| OCF183 |
C. elegans |
unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2] III; ieSi38 IV; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ltIs25; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ltIs25 [pie-1p::GFP::tba-2 + unc-119(+)]. ItIs25 is a translation fusion of GFP to unspliced genomic tba-2/C47B2.3. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurquoise2 tag with linker inserted at C-terminus of endogenous his-72 locus. 3xFLAG::AID* tag inserted at C-terminus of endogenous atln-1 locus. mCherry::SP12 is a stable germline and embryonic expression of ER and NE marker. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
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| RG5118 |
C. elegans |
atln-1(gk5537[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/tmC25 [unc-5(tm9708)] IV. Show Description
Maintain by picking wild-type GFP+. Apparent homozygous sterile deletion balanced over tmC25. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+ heterozgyotes, GFP+ gk5537 homozygotes (slow-growing, larval arrest/lethality), and GFP- Unc animals (tmC25 [unc-5(tm9708)] homozygotes). Derived from parental strains VC4464 and FX30257. gk5537 is a deletion of 4161 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking sequence: AATTCTCCATTATCCGGATTCTCGTGGCGT; Right flanking sequence: TGGAACGGTTGGAATCTGATTTGCAGGTAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.
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| VC4464 |
C. elegans |
atln-1(gk5537[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ IV. Show Description
Homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 4161 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: AATTCTCCATTATCCGGATTCTCGTGGCGT; Right flanking sequence: TGGAACGGTTGGAATCTGATTTGCAGGTAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.
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