| ciao-2B(mod334) III. Show Description
ciao-2B(mod334) is a CRISPR/Cas9-engineered missense variant [E137A]. Temperature-sensitive: maintain at 15-20C; reduced viability at 25C. Mild TMP/UVA-induced ICL sensitivity. Generated in N2 background. sgRNA: GGAACGAGTAGCTGCTGCAA. ssODN: GCCCCTCAAAAAGTTAATTTTTAAAACATAAATTCAAAATGTCTCATTTAGGACCGAGTAGCTGCTGCAATGGAAAATCAAGGACTAATGCACGCTGTTAACGAATGCCTCAGAGTTCCAGAA. Reference: Li et al., Nucleic Acid Res. 2024 Jul 16;52(16):9586–9595. doi: 10.1093/nar/gkae617. PMID: 41131702. |
