| nlp-42(syb235) V. Show Description
Complete CRISPR/Cas-9 knock-out (2317bp deletion) of the gene nlp-42(Y80D3A.10). Two times backcrossed with N2. Homozygous. Superficial wild-type.
Primers for crossing:
Fwd: cgagacttttaaccccgtcg
InFwd: aaagcccatgacttgctgaa
Rev: gctcaggtggttagagggtt
Wild-type bands: 580bp, 2652bp. Mutation band: 335bp.
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