PS611 |
C. elegans |
mab-21(sy155) III; him-5(e1490) V. Show Description
Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (95%). A 10th ray is found in about 50% of the sides scored. Body is slightly shorter. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
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PS8865 |
C. elegans |
lgc-42(sy1550) III. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of lgc-42;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: GAAATGGAAATCCGAAGGCTCCGCTGCAAGTGCA
Right flanking sequence: CTTTGGTTTTTATGTGGAGAGCTTGGGAAATTTCAG
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : GCTCCGCTGCAAGTGCACTT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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PS8882 |
C. elegans |
dmsr-11(sy1551) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-11;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CTGAGGCTGCAATTAATAACGGAATTGTTTCCTTCA
Right flanking sequence: TGGACGCATTAGTAAAgtaatttaaactttagc
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CTTTACTAATGCGTCCATGA
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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PS8884 |
C. elegans |
dmsr-14(sy1553) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-14;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: GATGTTTATTGCTGTTAGTGATTTCGGATGTGCAGT
Right flanking sequence: TACTGGTTTGATGCAATTATTTATAAGAAATTATTC
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : GATTTCGGATGTGCAGTTAC
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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PS8886 |
C. elegans |
gnrr-1(sy1555) I. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of gnrr-1;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: GTCAATGATTCTGTTCTATCAATTGTCTTCACAT
Right flanking sequence: ATTTGGCTTTATTTATTCTGGCATTTGTTGGAAATG
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : ATCAATTGTCTTCACATATT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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PS8888 |
C. elegans |
dmsr-5(sy1557) III. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-5;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: ctttgaaaaaaatggttgcagGGTCTACACAGTATTG
Right flanking sequence: CATCGGTATTTATGTCTTTTTGTGTGTTTTTTCG
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : GGGTCTACACAGTATTGCAT
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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PS8890 |
C. elegans |
dmsr-12(sy1559) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of dmsr-12;
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
Left flanking sequence: CCGGCCGTTTCACTACTATATATTCACTTCCTTAG
Right flanking sequence: TTGTTTTGCATTTTTTGCAAATATTATGATTGTG
inserted sequence between the two flanking sequence (STOP-In casette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA : CAAAAAATGCAAAACAACTA
Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
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