lgc-19(sy1472) IV. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of lgc-19.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: CTATCGATTCTTTTTTCTTTGATCCAGAGTTATAC
right flanking sequence: Ggtaggctattttggctttcaggctcaaaaaaggtg
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TTGATCCAGAGTTATACGGT
Method Reference: G3 (Bethesda).
|