| OH750 |
C. elegans |
otEx427. Show Description
otEx427 [hst-6p::GFP + rol-6(su1006)]. Transcriptional hst-6::GFP fusion. Maintain by picking Rollers.
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| OH7546 |
C. elegans |
vtIs1 V; otIs198. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. otIs198 [hsp16-2::ast-1 + ttx-3::DsRed + hsp16-2::NLS::mCherry]. Rollers. Heat shock induces expression of nuclear mCherry and AST-1. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
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| OH7547 |
C. elegans |
otIs199. Show Description
otIs199 [cat-2::GFP + rgef-1(F25B3.3)::DsRed + rol-6(su1006)]. Rollers. GFP expressed in dopaminergic neurons and dsRed expressed pan-neuronally. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
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| OH7687 |
C. elegans |
otEx3401. Show Description
otEx3401 [unc-14p::hif-1(P621A)::YFP(cDNA) + rol-6(su1006)]. Line 1. Pick Rollers.
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| OH7688 |
C. elegans |
otEx3402. Show Description
otEx3402 [unc-14p::hif-1(P621A)::YFP(cDNA) + rol-6(su1006)]. Line 2. Pick Rollers.
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| OH7689 |
C. elegans |
otEx3403. Show Description
otEx3403 [unc-14p::hif-1(P621A)::YFP(cDNA) + rol-6(su1006)]. Line 3. Pick Rollers.
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| OH7690 |
C. elegans |
otEx3398. Show Description
otEx3398 [unc-14p::hif-1::YFP(cDNA) + rol-6(su1006)]. Line 1. Pick Rollers.
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| OH7691 |
C. elegans |
otEx3399. Show Description
otEx3399 [unc-14p::hif-1::YFP(cDNA) + rol-6(su1006)]. Line 2. Pick Rollers.
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| OH7692 |
C. elegans |
otEx3400. Show Description
otEx3400 [unc-14p::hif-1::YFP(cDNA) + rol-6(su1006)]. Line 3. Pick Rollers.
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| OH7726 |
C. elegans |
otIs114 I; nhr-67(ot158) IV. Show Description
otIs114 contains [lim-6p::GFP + rol-6(su1006)]. Rollers.
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| OH7917 |
C. elegans |
ast-1(ot417) II; otIs199. Show Description
otIs199 [cat-2::GFP + rgef-1(F25B3.3)::DsRed + rol-6(su1006)]. Rollers. No cat-2::GFP expression in DA neurons (CEP, ADE, PDE). Maintain under normal conditions. Reference: Flames N, Hobert O, 2009 Nature 458, 885-889.
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| OH8001 |
C. elegans |
otIs114 I; lsy-12(ot177) V. Show Description
otIs114 [lim-6p::GFP + rol-6(su1006)]. Loss of lys-12 leads to the disruption of ASEL fate markers and the ectopic expression of ASER cell fate markers in ASEL. otIs114 reporter, normally expressed in ASEL and excretory gland cells, is lost in lsy-12 mutants. Rollers. Whole genome sequenced strain.
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| OH812 |
C. elegans |
otIs114 I. Show Description
otIs114 [lim-6p::GFP + rol-6(su1006)].
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| OH8545 |
C. elegans |
trp-4(ot477) I; vtIs1 vsIs33 V. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. vsIs33 [dop-3::RFP] V. Rollers. Whole genome sequenced strain.
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| OH8882 |
C. elegans |
otEx3909. Show Description
otEx3909 [ceh-36(fosmid)::YFP + rol-6(su1006)]. otEx3909 is a complex array derived from injection with PvuII-digested E. coli genomic DNA. ceh-36::YFP is broadly expressed in early stage embryos and becomes restricted to ASE and AWC later in development. Pick rollers to maintain.
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| OH8993 |
C. elegans |
otIs252. Show Description
otIs252 [lsy-6(fosmid)::YFP + rol-6(su1006)]. Rollers. YFP expression in ASEL.
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| OH911 |
C. elegans |
him-5(e1490) V; otIs35 X. Show Description
otIs35 [ttx-3p::kal-1 + rol-6(su1006)] X. otIs35 has been mapped to LG X between lon-2 and hst-6. Superficially wild-type; AIY interneurons have a 100% penetrant axon branching phenotype.
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| OH9305 |
C. elegans |
unc-37(ot240) otIs114 I. Show Description
otIs114 [lim-6p::GFP + rol-6(su1006)]. Rollers. Whole genome sequenced strain.
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| OH9345 |
C. elegans |
otEx4140. Show Description
otEx4140 [hlh-3(fosmid)::YFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. Reference: Murgan et al. (2015) Developmental Cell 33, 737-745.
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| OH9370 |
C. elegans |
otIs232; otEx4149. Show Description
otIs232 [che-1p::mCherry(C. elegans-optimized)::che-1 3'UTR + rol-6(su1006)]. otEx4149 [cog-1(fosmid)::YFP + elt-2::DsRed]. Maintain by picking animals dsRed(+) in gut nuclei. Rollers. mCherry expression in ASER and ASEL.
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| OH9482 |
C. elegans |
lol-1(ot567); otIs138 X. Show Description
otIs138 [ser-2(prom3)::GFP + rol-6(su1006)] X. Integrated array derived from otEx449. Whole genome sequenced strain.
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| OH9545 |
C. elegans |
otIs287 IV. Show Description
otIs287 [rab-3(prom1)::2xNLS::YFP + rol-6(su1006)] IV. Rollers. Pan-neuronal YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH96 |
C. elegans |
him-5(e1467) V; mgIs19 X. Show Description
Rollers. Throws males. mgIs19 [lim-4::GFP + rol-6(su1006)].
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| OH9609 |
C. elegans |
otIs291 V. Show Description
otIs291 [rab-3(prom1)::2xNLS::YFP + rol-6(su1006)] V. Rollers. Pan-neuronal YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH9625 |
C. elegans |
otIs292. Show Description
otIs292 [eat-4::mCherry + rol-6(su1006)]. Rollers. Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8.
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| OH9671 |
C. elegans |
otIs114 I; lsy-27(ot108) II; otIs220 IV. Show Description
otIs220 [gcy-5::mCherry]. otIs114 [lim-6p::GFP + rol-6(su1006)]. Rollers. 2 ASER.
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| OH9838 |
C. elegans |
otIs232; otEx4359. Show Description
otIs232 [che-1p::mCherry(C. elegans-optimized)::che-1 3'UTR + rol-6(su1006)]. Rollers. mCherry expression in ASER and ASEL. otEx4359 [die-1(prom8)::2NLS::YFP + elt-2::DsRed]. Pick animals with dsRed+ intestinal nuclei to maintain otEx4359. otEx4359 carries a minimal (1 kb) promoter driving 2NLS::YFP only in ASEL.
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| OH9846 |
C. elegans |
otIs305 ntIs1 V. Show Description
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp16-2p::che-1::3xHA::BLRP + rol-6(su1006)] V; injected as complex array with Pvu II bacterial DNA. Rollers. Rol is prone to silencing in otIs305, but hsp transgene is still active in Hobert Lab assays. otIs305 is homozygous by PCR analysis (Hobert). Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8.
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| OH9882 |
C. elegans |
otEx4390. Show Description
otEx4390 [8xASE::GFP::cog-1 3'UTR + rol-6(su1006)]. Maintain by picking Rollers.
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| OH998 |
C. elegans |
otIs114 I; lin-49(ot78) IV; him-5 V. Show Description
otIs114 [lim-6p::GFP + rol-6(su1006)]. Rollers. Him. 2 ASER. Whole genome sequenced strain.
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| OH9991 |
C. elegans |
otIs114 I; otIs220 IV; lsy-12(ot563) ntIs1 V. Show Description
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs220 [gcy-5::mCherry]. otIs114 [lim-6p::GFP + rol-6(su1006)]. Rollers. 2 ASER.
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| OH9995 |
C. elegans |
otIs313. Show Description
otIs313 [sem-2(fosmid)::YFP + rol-6(su1006)]. Rollers. Reference: Vidal B, et al. Development. 2015 Jul 15;142(14):2464-77.
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| OK29 |
C. elegans |
cuIs1 V. Show Description
cuIs1 [ceh-22::GFP + rol-6(su1006)]. Rollers.
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| OK39 |
C. elegans |
cuIs2 IV. Show Description
cuIs2 [myo-2c:: GFP + rol-6(su1006)]. Rollers.
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| OK66 |
C. elegans |
daf-4(m72) III; cuIs2/+ IV. Show Description
cuIs2 [myo-2c:: GFP + rol-6(su1006)]. daf-4(m72) is a temperature sensitive dauer constitutive mutant. Maintain at 15C. It was problematic to get cuIs2 homozygous in this background. Pick Rollers to maintain.
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| OK68 |
C. elegans |
cuIs2 IV; daf-3(mgDf90) X. Show Description
Dauer defective. cuIs2 [myo-2c:: GFP + rol-6(su1006)]. Rollers. [NOTE: this strain was originally described as carrying daf-3(mg90), which is an old name for daf-3(mgDf90).]
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| OS132 |
C. elegans |
nsEx37. Show Description
nsEx37 [cfi-1::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. Reference: Shaham S, Bargmann CI. Genes Dev. 2002 Apr 15;16(8):972-83.
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| OS1579 |
C. elegans |
nsEx858. Show Description
nsEx858 [F16F9.3p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Specific expression of GFP in AMsh. Reference: Bacaj T, et al. Science. 2008 Oct 31;322(5902):744-7.
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| OS1585 |
C. elegans |
nsEx864. Show Description
nsEx864 [F11C7.2p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Specific expression of GFP in AMsh. Reference: Bacaj T, et al. Science. 2008 Oct 31;322(5902):744-7.
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| OS1776 |
C. elegans |
hsf-1(sy441) I; nsEx992. Show Description
nsEx992 [vap-1::hsf-1 + hsp::FRT-GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. GFP expression in amphid sheath cells and occasionally gut following heat shock. Reference: Bacaj T, Shaham S. Genetics. 2007 Aug;176(4):2651-5.
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| OS1889 |
C. elegans |
nsEx1066. Show Description
nsEx1066 [F53F4.13p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Specific expression of GFP in AMsh. Reference: Bacaj T, et al. Science. 2008 Oct 31;322(5902):744-7.
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| OS1918 |
C. elegans |
nsEx1086. Show Description
nsEx1086 [T02B11.3p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Specific expression of GFP in AMsh. Reference: Bacaj T, et al. Science. 2008 Oct 31;322(5902):744-7.
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| OS2017 |
C. elegans |
hsf-1(sy441) I; nsEx1129. Show Description
nsEx1129 [osm-6p::hsf-1 + hsp-16-2::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. GFP expression in amphids and phasmids following heat shock; some background expression in pharyngeal muscle cells. Reference: Bacaj T, Shaham S. Genetics. 2007 Aug;176(4):2651-5.
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| OS2723 |
C. elegans |
hsf-1(sy441) I; che-2(e1033) X; nsEx1552. Show Description
nsEx1552 [sra-6p::hsf-1 + hsp-16-2::che-2 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. Reference: Bacaj T, Shaham S. Genetics. 2007 Aug;176(4):2651-5.
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| OS2728 |
C. elegans |
hsf-1(sy441) I; che-2(e1033) X; nsEx1555. Show Description
nsEx1555 [osm-6p::hsf-1 + hsp-16-2::che-2 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. Reference: Bacaj T, Shaham S. Genetics. 2007 Aug;176(4):2651-5.
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| OS3062 |
C. elegans |
hsf-1(sy441) I; nsEx1730. Show Description
nsEx1730 [myo-2p::hsf-1 + hsp-16.2::GFP + hsp-16.41::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. Pharyngeal GFP expression following heat-shock. Reference: Bacaj T, Shaham S. Genetics. 2007 Aug;176(4):2651-5.
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| OU247 |
C. elegans |
zyIs1. Show Description
zyIs1 [lin-11::RFP + rol-6(su1006)]. Roller. Reference: Sanchez-Alvarez L, et al. PLoS Genet. 2011 Sep;7(9):e1002257.
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| OU6 |
C. elegans |
png-1(cy9) I; cyIs4. Show Description
cyIs4 [cat-1::GFP + rol-6(su1006)]. Reference: Habibi-Babadi N, et al. J Neurosci. 2010 Feb 3;30(5):1766-76.
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| PC71 |
C. elegans |
ubIs4. Show Description
ubIs4 [hsp16.1::hsp-16A::lacZ + rol-6(su1006)]. Transgene contains the complete hsp16.48-1 gene pair of locus hsp16A with lacZ cloned in-frame into the second exon of hsp16.1. The contruct contains the SV40 nuclear localization signal fused to the beginning of the lacZ coding region. Published as ubIn4.
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| PC72 |
C. elegans |
ubIs5. Show Description
ubIs5 [hsp16.1::hsp-16A::lacZ + rol-6(su1006)]. Transgene contains the complete hsp16.48 and hsp16-1 gene pair of locus hsp16A with lacZ cloned in-frame into the second exon of hsp16.1. The contruct contains the SV40 nuclear localization signal fused to the beginning of the lacZ coding region. Published as ubIn5.
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