goeIs431 [hsp-16.2p::nlp-25::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of nlp-25::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791

axIs1569 [pie-1p::GFP::msp-142 ORF::msp-142 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.

axIs1673 [pie-1p::GFP::histone H2B::msp-142 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.

axIs1759 [msp-56p::GFP::histone H2B:tbb-2 3'utr + unc-119(+)].

qIs56 [lag-2p::GFP + unc-119(+)] V. qEx557 [hsp-16p::ceh-22b + ttx-3::DsRed]. Him. Pick DsRed+ animals to maintain qEx557 array. Heat-shock can be used to drive the ectopic expression of CEH-22B (derived from Fire Lab vector pPD49.78). LAG-2::GFP is expressed the embryo, nerve cord, Z1/Z4, and DTCs. Reference: Lam N. et al. Curr Biol. 2006 Feb 7;16(3):287-95. doi: 10.1016/j.cub.2005.12.015. PMID: 16461282

No apparent phenotype. tm748 is a UV/TMP-induced gap-2 deletion allele generated by National Bioresource Project (see http://shigen.lab.nig.ac.jp/c.elegans/index.jsp?lang=englis h for info).

peIs1713 [sra-6p::mCasp-1 + unc-122p::mCherry]. ASH neurons are eliminated. Abnormal odor chemotaxis. Reference: Yoshida K, et al. Nat Commun. 2012 Mar 13;3:739.

peIs1715 [str-1p::mCasp-1 + unc-122p::GFP]. AWB neurons are eliminated. Abnormal odor chemotaxis. Reference: Yoshida K, et al. Nat Commun. 2012 Mar 13;3:739.

Recessive Daf-c mutation that also causes Odr and Che defects. Maintain at 15C. [The mec-1 mutation present in the original isolates has been eliminated.] Previously known as daf-21.

Caenorhabditis sp. 7 Male-female strain. Isolated by Marie-Anne Felix from rotting citrus fruit sampled by M. Herrmann in Begoro, Ghana in June 2007. New male-female species of the elegans group. Does not produce cross-progeny with any of the other previous elegans group species. Isofemale line. Maintain by mating.

Caenorhabditis sinica wild isolate. Caenorhabditis sp. 5 Male-female strain. Isolated from a small fruit found in the Garden of Harmony in Suzhou, China, on August 17, 2007.

Caenorhabditis sinica wild isolate. Caenorhabditis sp. 5 Male-female strain. Isolated from a small fruit found in the Feilaifeng Park in Hangzhou, China, on August 25, 2007. Similar fruit as JU1201.

Caenorhabditis sp. 7 Male-female strain. Caenorhabditis sp. 7 is currently an undescribed species. The sequenced strain, JU1286, is an isogenic inbred line derived from strain JU1199 by 20 consecutive matings of 1 virgin female and 1 male. The inbreeding was done by Marie-Anne Felix. Strain JU1199 was isolated in June 2007 by Matthias Herrmann from a rotting citrus fruit in Begoro, Ghana, Africa. C. sp. 7 is a gonochoristic species with about equal proportions of males and females.

Caenorhabditis sp. 9 Male-female strain. Isolated from rotting flowers and leaves sampled in the Zoo/Botanical Garden of Trivandrum, Kerala, India on 21 Dec 2007. Culture at 20°C or above. Reference: Félix, Braendle & Cutter, 2014

Caenorhabditis sp. 10 Male-female strain. Isolated by Marie-Anne Félix from rotting cacao fruit sampled in Angela Spice Garden a few kilometers from Periyar, Kerala, India on 27 Dec 2007. Culture at 20°C or above.

Caenorhabditis sp. 11 Isolated by Marie-Anne Felix from rotting torch ginger (Etlingeria elatior) flowers sampled in the island of La Réunion by Valérie Robert and Loïc Sablé in Jan 2008. Hermaphrodite. Culture at 20°C or above.

Caenorhabditis sp. 9 Male-female strain. Derived by 25 rounds of inbreeding (1 virgin female + 1 male) from JU1325, isolated from rotting flowers and leaves sampled in the Zoo/Botanical Garden of Trivandrum, Kerala, India on 21 Dec 2007. Culture at 20°C or above.

Caenorhabditis sp. 12 Male-female strain. Isolated in Nouragues, French Guiana. Reference: Kiontke KC, et al. BMC Evol Biol. 2011 Nov 21;11:339.

Caenorhabditis sp. 12 Male-female strain. Isolated in May 2008 from rotting Micropholis cayennensis fruit (#1, subsample L), sampled by P. Châtelet on the "Petit Plateau" near the CNRS Biological station, Nouragues, French Guyana.

Caenorhabditis sp. 11 Isolated by Marie-Anne Felix from rotting Duguetia surinamensis fruit, sampled by Patrick Châtelet on the "Petit Plateau" in the Nouragues Forest, French Guyana in May 2008. Hermaphrodite. Culture at 20°C or above.

Caenorhabditis sp. 7 Male-female strain. Isolated from leaf litter near pond edge, sampled by J. and G. Hatty on 29 Nov 08 near Shonga, Nigeria.

Inbred line from wild isolate SB341. Inbred for 20 generations by sib mating L4 female and male. Previously known as Caenorhabditis sp. 1.

Caenorhabditis sp. 10 Male-female strain. Inbred line from JU1333. Inbred for 25 generations by brother-sister (L4) mating.

Caenorhabditis sp. 17. Maintain at 23C. Isolated from rotten wide bean of Barokia sp. sampled on Grand Plateau in Nouragues Forest, French Guiana, on 21 Nov 2009. Reference: Félix, Braendle & Cutter, 2014

Caenorhabditis sp. 18 Male-female strain. Isolated in Nouragues, French Guiana. Reference: Kiontke KC, et al. BMC Evol Biol. 2011 Nov 21;11:339.

Caenorhabditis sp. 16 25X inbred derivative of JU1873. Use for genome sequencing.

Caenorhabditis sp. 14 Male-female strain. Isolated in Petit-Bourg, Guadeloupe. Reference: Kiontke KC, et al. BMC Evol Biol. 2011 Nov 21;11:339.

Caenorhabditis sp. 13 Male-female strain. Inbred derivative of JU1528. Derived by sib mating (1 virgin female + 1 male) for 25 generations.

Caenorhabditis sp. 17 Male-female strain. Isogenic wild type line derived from JU1825. 28 rounds of sib mating with virgin females. Use as reference.

Caenorhabditis sp. 18 Male-female strain. Isogenic wild type line derived from JU1857. 25 rounds of sib mating with virgin females.

Isolated by M.-A. Félix from rotting fruits of Calophyllum inophyllum (red mahogany) sampled in the Jozani National Forest, Zanzibar, Tanzania (6.2715°S, 39.416°E), on 3/9/2012. Previously known as Caenorhabditis sp. 26.

Male-Female strain. Isolated by M.-A. Félix from a rotting mandarin sampled on a farm in the Dole district, Zanzibar, Tanzania (6.1074°S, 39.2515°E) , on 3/7/2012. Previously known as Caenorhabditis sp. 26.

Male-Female strain. 26 rounds of sib mating (L4 female) of JU2161. Healthy. Use for genome sequencing. Previously known as Caenorhabditis sp. 26.

Male-female strain. From a rotting kumquat-like fruit collected by Ludmilla Lokmane in Madre de Dios (Peru) in Jan 2013. Isolated by Lise Frézal. Line started from an adult isolated on 9 Jan 2013. Previously known as Caenorhabditis sp. 31.

Male-female strain. Inbred line derived from JU2469 by 25 rounds of brother-sister mating using a L4 female larva and a male. Healthy. Use for genome sequencing. Previously known as Caenorhabditis sp. 31.

Male-female strain. Isolated from a rotting fruit sampled on 09 June 2014 by Fabrice Besnard in Guadeloupe (Basse Terre, 16.1761, -61.6951). Started with a plugged female. Previously known as Caenorhabditis sp. 38.

Isolated from humus sampled on 08 August 2014 below the cathedral fig tree Ficus destruens by Danbulla Road, Australia (-17.1774, 145.6600) by Jean-Baptiste Pénigault. Started on 19 August from a plugged female. Crossed with sp. 5 JU727 in both directions; the outcome is more than 100 embryos, no larvae. Previously known as Caenorhabditis sp. 40.

Inbred line derived from SB454 by 25 rounds of brother-sister mating using a L4 female larva and a male. Healthy. Use for genomic sequencing. Previously known as Caenorhabditis sp. 32.

Inbred line derived from JU2745 by 25 rounds of brother-sister mating using a L4 female larva and a male. Used for DNA/RNA sequencing. Previously known as Caenorhabditis sp. 38.

Inbred line derived from JU2774 by 25 rounds of brother-sister mating using a L4 female larva and a male. Used for DNA/RNA sequencing. Previously known as Caenorhabditis sp. 40.

C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from destroyed snail Oxychilus sp.

From Merlet, Lagorce (Ardèche), France. 8 Sep 02. From a Oxychilus sp. snail , under the mulberry tree.

Sluggish Unc. Recessive autosomal. Parental strain Oscheius tipulae CEW1. AKA Oscheius sp. 1.

Male-female strain. Isolated on May 6, 2005 under a tree along a path between two villages in Changyang, 20 km north of Sanjiang, Guangxi, China. Does not cross with C. remanei PB4641 or JU724, nor with C. sp. PB2801. sp. 5 in Kiontke and Sudhaus Wormbook Ecology chapter.

Male-female strain. Inbred derivative of JU727. Derived from JU727 by 20 generations of sib mating. Male/female strain. Isolated on May 6, 2005 under a tree along a path between two villages in Changyang, 20 km north of Sanjiang, Guangxi, China. Does not cross with C. remanei PB4641 or JU724, nor with C. sp. PB2801. sp. 5 in Kiontke and Sudhaus Wormbook Ecology chapter.

Bacteria. CeMbio Collection. Natural isolate from C. elegans natural habitat (Rotting apple). LB, 20-26C. Sampled in Santeuil, France. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: ATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATGCAAGCCGAGCGGTAGAGATCTTTCGGGATCTTGAGAGCGGCGTACGGGTGCGGAACACGTGTGCAACCTGCCTTTATCAGGGGGATAGCCTTTCGAAAGGAAGATTAATACCCCATAATATATTGAATGGCATCATTTGATATTGAAAACTCCGGTGGATAGAGATGGGCACGCGCAAGATTAGATAGTTGGTAGGGTAACGGCCTACCAAGTCAGTGATCTTTAGGGGGCCTGAGAGGGTGATCCCCCACACTGGTACTGAGACACGGACCAGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGATCCAGCCATCCCGCGTGAAGGACGACGGCCCTATGGGTTGTAAACTTCTTTTGTATAGGGATAAACCTTTCCACGTGTGGAAAGCTGAAGGTACTATACGAATAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTAGGCGGATCTGTAAGTCAGTGGTGAAATCTCATAGCTTAACTATGAAACTGCCATTGATACTGCAGGTCTTGAGTAAAGTAGAAGTGGCTGGAATAAGTAGTGTAGCGGTGAAATGCATAGATATTACTTAGAACACCAATTGCGAAGGCAGGTCACTATGTTTTAACTGACGCTGATGGACGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGCTAACTCGTTTTTGGGTCTTCGGATTCAGAGACTAAGCGAAAGTGATAAGTTAGCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGATTATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCAAGGCTTAAATGGGAATTGACAGGTTTAGAAATAGACTTTTCTTCGGACAATTTTCAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTTAGGTTAAGTCCTGCAACGAGCGCAACCCCTGTCACTAGTTGCCATCATTCAGTTGGGGACTCTAGTGAGACTGCCTACGCAAGTAGAGAGGAAGGTGGGGATGACGTCAAATCATCACGGCCCTTACGCCTTGGGCCACACACGTAATACAATGGCCGGTACAGAGGGCAGCTACCTAGCGATAGGATGCGAATCTCGAAAGCCGGTCTCAGTTCGGATTGGAGTCTGCAACTCGACTCTATGAAGCTGGAATCGCTAGTAATCGCATATCAGCCATGATGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGGAAGTTTGGGGTACCTGAAGTCGGTGACCGTAACAGGAGCTGCCTAGGGTAAAACAAGTAACTAGGGCTAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAACATCTCATT

oxSi120 [peel-1p::tagRFP::MSP-142 3'utr+ unc-119(+)]. Him. Derived from EG5897 and CB1489; not known if is unc-119(ed3) is still present in background. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34.

huIs120 [hsp-16.2p::sfrp-1 + myo-2p::Tomato]. Wild-type under normal culture conditions. Heat-shock (10 min) during embryogenesis causes embryonic lethality and HSN migration defects. Heat-shock (10 min) at hatching causes Ql and QR migration defects. Reference: Harterink M, et al. Development. 2011 Jul;138(14):2915-24.

huIs4 [hsp-16.2p::pop-1(delta N) + rol-6(su1006)]. Rollers. Truncated pop-1 fragment encoding delta N-POP-1(45-438) driven by heat-shock promotor hsp-16.2. Reference: Korswagen et al. (2000) Nature 406: 527-32.

huIs7 [hsp-16.2p::Myc::bar-1(delta N) + mec-7p::GFP + dpy-20(+)]. Muv. Posterior migration of the QR descendants. Reference: de Groot et al. (2014) Science Signal. Ra26.