| RW12244 |
C. elegans |
sox-2(st12244[sox-2::TY1::EGFP::3xFLAG]) X. Show Description
CRISPR/Cas9 engineered tagged endogneous locus.
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| NL713 |
C. elegans |
mut-2(r459) I; sox-2(pk65). Show Description
K08A8.2. Location of the insertion: TCACGTATCT TA CATATTATAT.
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| OH10051 |
C. elegans |
unc-119(ed3) III; sox-2(ot640[unc-119(+)])/+ X. Show Description
ot640 was generated by using MosDel removing the entire sox-2 locus and insert unc-119(+). Heterozygotes are WT and segregate WT heterozygotes, Uncs and ot640 homozygous that arrest in L1 with a pharynx unattached (Pun) phenotype. Maintain by picking WT and check for correct segregation of progeny to maintain. Reference: Vidal B, et al. Development. 2015 Jul 15;142(14):2464-77.
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| OH10053 |
C. elegans |
unc-119(ed3) III; sox-2(ot640[unc-119(+)]) X; otEx4454. Show Description
otEx4454 [sox-2(fosmid)::mCherry + elt-2p::DsRed]. ot640 was generated by using MosDel removing the entire sox-2 locus and insert unc-119(+). ot640 homozygous arrest in L1 with a pharynx unattached (Pun) phenotype. Maintain by picking WT to maintain (transgene should be required for viability). Reference: Vidal B, et al. Development. 2015 Jul 15;142(14):2464-77.
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| OH10330 |
C. elegans |
otIs333 II. Show Description
otIs333 [sox-2(fosmid)::mCherry + rol-6(su1006)]. Rollers. Reference: Vidal B, et al. Development. 2015 Jul 15;142(14):2464-77.
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| RW11425 |
C. elegans |
unc-119(tm4063) III; stIs11425. Show Description
stIs11425 [sox-2.b::H1-wCherry + unc-119(+)].
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| SYS516 |
C. elegans |
ujIs113 II; sox-2(dev153([sox-2::mNeonGreen]) X. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::mCherry::his-24::let-858 3Â’UTR + unc-119(+)] II. mNeonGreen knockin at C-terminus of sox-2 locus. Cellular protein expression pattern during embryogenesis (until bean stage) is available at http://dulab.genetics.ac.cn/TF-atlas. Reference: Ma X, Zhao Z, Xiao L, et al. Nat Methods. 2021;18(8):893-902. doi:10.1038/s41592-021-01216-1.
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