More Fields
Strain Species Genotype
RB1882 C. elegans sgn-1(ok2432) II. Show Description
F07H5.2. Homozygous. Outer Left Sequence: ACGTGTGTTTGTGGGTTTCA. Outer Right Sequence: AAAGCCACGTTCGATTCAAG. Inner Left Sequence: CCCCTTCTCAATATTCGGGT. Inner Right Sequence: AAGCTTCCAGCCTCCTTTGT. Inner Primer PCR Length: 3103 bp. Deletion Size: 1620 bp. Deletion left flank: TTGAGCACAATTCCGATGGCAAGGATCAAA. Deletion right flank: AGGTTTAATTATATTATTATAGTACACATC. Insertion Sequence: TATATTATTATAGTACAC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC1165 C. elegans let-19&sgn-1(ok331)/mIn1 [mIs14 dpy-10(e128)] II. Show Description
F07H5.2, K08F8.6. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok331 homozygotes (grotty sterile Dpy with vulval blip). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TGAAAATTGGGAGTTCGGAG. External right primer: ACCACTTGTTCCTTGCCAAC. Internal left primer: GGACTGGAAACTCCAAGCAG. Internal right primer: GACTGATGAGCCGGTATGGT. Internal WT amplicon: 2637 bp. Deletion size: 1456 bp. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807