OH2246 |
C. elegans |
otIs107. Show Description
otIs107 [ser-2::GFP + lin-15(+)]. Not on LG X.
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OH313 |
C. elegans |
ser-2(pk1357) X. Show Description
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RB1690 |
C. elegans |
ser-2(ok2103) X. Show Description
C02D4.2. Homozygous. Outer Left Sequence: CTCCTTCGCCACACTGATTT. Outer Right Sequence: CCGAATCGCGAAAATTAAAA. Inner Left Sequence: CTTTCACTTTGCACGATCCA. Inner Right Sequence: TCCGCAAATTTCTACGATCC. Inner Primer PCR Length: 3130 bp. Deletion Size: 2043 bp. Deletion left flank: GTTTGTGTCATCAACTAAAAAAATAACTAA. Deletion right flank: GACAATGATCATTAACTTAATGAGCTTTCC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
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OH1360 |
C. elegans |
bgIs312; otEx718. Show Description
bgIs312 [pes-6::GFP]. GFP expression in excretory cell only. otEx718 [exc-4p::exc-4::DsRed2 + rol-6(su1006)]. Maintain by picking Rollers. Roller phenotype most penetrant at 20C.
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OH3314 |
C. elegans |
oyIs14 V; otIs38 X. Show Description
oyIs14 [sra-6::GFP + lin-15(+)]. otIs38 [unc-47(del)::GFP]. otIs38 is integrated juEx60 [(pCZ114) unc-47(del)::GFP]. GFP fluorescence in PVT and DBV.
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JN3038 |
C. elegans |
qjIs11; peIs3042; peIs2100; qjIs14. Show Description
qjIs11 [glr-1p::SVNLS2::TagBFPsyn + ser-2(prom2)::SVNLS2::TagBFPsyn]. peIs3042 [eat-4p::svnls2::TagRFP675syn + lin-44p::GFP]. peIs2100 [H-20p::NLS4::mCherry]. qjIs14 [H20p::NLS::YC2.60]. Suitable for whole-brain calcium imaging. mCherry and YC2.60 expression in almost all head neurons. BFP and RFP are also expressed to annotate neurons. H20p is a pan-neuronal promoter expressed in almost all neurons, GLR glial cells, XXX hypodermal cells, pharyngeal gland cells and HMC cells. Reference: Toyoshima Y et al. BMC Biol. 2020 Mar 19;18(1):30. PMID: 32188430; Shioi G, et al. Genetics. 2001 Apr;157(4):1611-22. PMID: 11290717.
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NC3182 |
C. elegans |
otIs181 III; otIs138 X; otIs396. Show Description
otIs181[dat-1::mCherry + ttx-3::mCherry] III. otIs138[ser-2(prom3)::GFP + rol-6(su1006)] X. otIs396 [ace-1prom2::NLS::tagRFP]. Rollers. dat-1::mCherry labels ADE, CEP, and PDE neurons. ttx3::mCherry labels AIY neurons. ser-2(prom3)::GFP labels OLL, PDE, and PVD neurons.
ace-1prom2::NLS::tagRFP labels CEP and OLL neurons. PVD can be identified by expression only GFP. An additional pair of GFP-only cells anterior to OLL are occasionally observed in this strain. Can be used to isolate PVD by FACS (green-only). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). Reference: Barbara OBrien (2017) Diverse genetic and transcriptional programs mediate dendritic development of a nociceptor neuron. Ph.D Dissertation, Vanderbilt University. (https://ir.vanderbilt.edu/handle/1803/14489?show=full)
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OH10765 |
C. elegans |
pha-1(e2123) III; otIs358. Show Description
otIs358 [ser-2(prom2)::GFP + pha-1(+)].
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OH11166 |
C. elegans |
vab-3(ot569); otIs138. Show Description
otIs138 [ser-2(prom3)::GFP + rol-6(su1006)]. Rollers. Reference: Serrano-Saiz E, et al. Cell 2013. Oct; 155(3):659-73.
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OH1422 |
C. elegans |
otIs138 X. Show Description
otIs138 [ser-2(prom3)::GFP + rol-6(su1006)] X. Integrated array derived from otEx449.
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OH16719 |
C. elegans |
otIs138 X; otIs521. Show Description
otIs138 [ser-2(prom3) +rol-6(su1006)] X. otIs521 [eat-4(prom8)::tagRFP + ttx-3::GFP]. OLL neurons are labeled with GFP and RFP. Can be used to isolate OLL by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
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OH898 |
C. elegans |
pha-1(e2123) III; otEx536. Show Description
otEx536 [ser-2(prom2)::GFP + pha-1(+)]. Maintain at 25C to select for transgenic animals.
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OH9482 |
C. elegans |
lol-1(ot567); otIs138 X. Show Description
otIs138 [ser-2(prom3)::GFP + rol-6(su1006)] X. Integrated array derived from otEx449. Whole genome sequenced strain.
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SRS306 |
C. elegans |
pha-1(e2123) III; lite-1(ce314) X; sraEx306. Show Description
sraEx306 [ser-2(prom2)::chop-2(H134R)::TagRFP + ser-2(prom2)::mKO + pBX(pha-1(+))]. Maintain at 25C. This transgenic line expresses mKO and TagRFP in AIY, AIZ and RME in the head, and has little response to blue light in the absence of ATR. In the presence of ATR asymmetrical stimulation of AIY causes the worm to turn in the same direction the head was bent when AIY was excited, whereas asymmetrical stimulation of AIZ or RME causes the worm to turn in the opposite direction to which the head was bent when AIZ or RME was excited. Reference: Kocabas A, et al. Nature. 2012 Sep 23. doi: 10.1038/nature11431.
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