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Strain Species Genotype
EW35 C. elegans sem-4(ga82) I. Show Description
Egl.
MH1346 C. elegans kuIs35. Show Description
kuIs35 [sem-4::GFP].
MT3179 C. elegans sem-4(n1378) I. Show Description
Sex muscles are absent. Egl.
MT5825 C. elegans sem-4(n1971) I. Show Description
Egl - Bag, Con. Strong allele of sem-4.
MT6921 C. elegans sem-4(n2654) I. Show Description
Aex. Con.
MT3184 C. elegans sem-4(n1378) unc-13(e51) I. Show Description
Unc. Egl. Transformation of sex myoblasts into body wall muscle.
MT3213 C. elegans dpy-5(e61) sem-4(n1378) I. Show Description
Dpy. Egl. Transformation of sex myoblasts into body wall muscle.
MT3248 C. elegans bli-4(e937) sem-4(n1378) I. Show Description
MT5884 C. elegans sem-4(n1971) I; unc-49(e382) III. Show Description
Egl. Unc-shrinker.
MT6505 C. elegans sem-4(n1378) I; unc-30(e165) IV. Show Description
MT7169 C. elegans sem-4(n1378) I; ced-5(n2098) ced-3(n2720) IV. Show Description
MT7170 C. elegans sem-4(n1378) I; ced-5(n2098) ced-3(n2719) IV. Show Description
MT7502 C. elegans sem-4(n1378) I; ced-5(n2098) ced-3(n2830) IV. Show Description
CZ20215 C. elegans tba-1(ju89) I. Show Description
Gain-of-function allele isolated sem-4; juIs1 screen in 1997. ju89 has slightly semi-dominant effects, and the gf phenotype is most obvious in homozygous. This outcrossed strain does not carry a sem-4 mutation or juIs1. References: Kurup N, et al. Curr Biol. 2015 Jun 15;25(12):1594-605. Kurup N, et al. PLoS Genet. 2017 Jun 21;13(6):e1006844.
MH1337 C. elegans kuIs34 IV. Show Description
kuIs34 [sem-4::GFP + unc-119(+)] IV. Superficially wild-type. Partial translational fusion reporter containing approximately half of SEM-4 fused in-frame to GFP. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785. Grant K, et al. Dev Biol. 2000 Aug 15;224(2):496-506.
NFB1899 C. elegans vlcEx1093. Show Description
vlcEx1093 [sem-4p::GFP::unc-54 3'UTR + rol-6(su1006)]. Not all transgenic animals roll well; pick GFP+ or Rollers to maintain. Reporter contains sem-4 enhancer sequence (+3376, +4551) inserted into pDD95.75. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785.
OP57 C. elegans unc-119(ed3) III; wgIs57. Show Description
wgIs57 [sem-4::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)