Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs. cyk-1 was previously known as let-794 (in Stewart et al 1998).

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs. pka let-833(s2837).

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs. DpyUncs die as embryos.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs.

Maintain by picking Uncs. DpyUncs arrest at early-mid larval stages.

Maintain by picking Unc-32 animals. Dpy animals that have lost sDp3 should also be Mel; expected Mel phenotype is embryonic lethal.

Throws Unc-36 and WT. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.

svDp1 [sur-5::GFP] (III;f). svDp1 was derived by insertion of a sur-5::GFP marker into sDp3(III;f). Pick GFP+ wild-type to maintain. svDp1 rescues ct224. ct224 homozygotes (lacking GFP expression) show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. The duplication is lost in about 30-40% of embryos. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.

May have unc-4(e120) mutation in background. svDp1 balances from pal-1 through unc-36 on III. svDp1 made by fusing array containing [unc-4(+) + sur-5::GFP] to sDp3(III;f).

WT sDp3-bearing animals segregate WT and lon-1 par-3 homozygotes that lay eggs that don't hatch. par-3 exhibits mildly ts penetrance in homozygotes. Strict maternal effect. Received new stock 3/01.

Animals with the Duplication are WT. Animals which have lost the Duplication are Lon and throw dead eggs.

Animals with the duplication are WT. Animals which have lost the duplication arrest at 3rd larval stage with increased life span. ua2 is a deletion of the first 2 exons of atp-2. atp-2 gene encodes for active site subunit of Complex V of mitochondrial respiratory chain, the ATP synthase.

Superficially WT. Pick WT to propagate. Throws WT (expressing unc-119::YFP) and DpyUncs. irDp1 is sDp3 carrying a spontaneous integrant of an array carrying unc-119::YFP + unc-32(+) + med-1(+), originally generated in BC4638. irDp1 appears to complement everything sDp3 does and has a similar meiotic transmission frequency of 60%. In another strain, irDp2 was observed to lose ability to complement dpy-17. irDp1 confers a progressive adult egg laying defect (also seen with sDp3).

Pick WT to maintain strain. Dp lost at high frequency, usable for mosaic analysis. Animals without the Dp are Unc, Vul and Ncl.

WT strain which throws WT and dead eggs. Sick and slow growing!!!

Unc strain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.