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Strain Species Genotype
DA949 C. elegans bli-6(sc16) egl-19(n582) unc-24(e138) IV. Show Description
Blistered cuticle. Egl-Lon, slow and floppy. Unc.
DH1033 C. elegans sqt-1(sc103) II; bIs1 X. Show Description
bIs1[vit-2::GFP + rol-6(su1006)].
DR1367 C. elegans bli-6(sc16) mec-3(e1338) IV. Show Description
Blistered. Mechanosensory abnormal.
EG1020 C. elegans bli-6(sc16) IV; dpy-11(e224) V; lon-2(e678) X. Show Description
Dpy suppresses Bli and Lon. Strain appears to be only slightly Dpy. Useful for mapping, especially Unc mutations. Separately, dpy-11 causes dumpiness, bli-6 adult worms develop blisters on their bodies, and lon-2 worms are about 150% WT length.
EH135 C. elegans unc-44(e362) bli-6(sc16) IV. Show Description
Blistered. Unc.
EW49 C. elegans unc-30(e191) IV; deIs12. Show Description
deIs12[bar1p::GFP + pSC11(unc-30(+))]. Green worms that are non-Unc.
IA260 C. elegans unc-51(e369) rol-9(sc148)/sec-23(ij13) V. Show Description
Heterozygotes are WT and segregate WT, Roller Unc and embryonic lethals.
JK1521 C. elegans fog-2(q71) pha-4(q490)/stu-3(q265) rol-9(sc148) V. Show Description
Heterozygotes are WT and segregate WT, Females which lack a pharynx (arrest as embryos or L1) and Sterile Unc Rollers. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. See also WBPaper00003770.
JR2750 C. elegans bli-6(sc16) unc-22(e66)/unc-24(e138) vha-17(w13) dpy-20(e2017) IV. Show Description
Pick wild type animals to maintain.
KJ487 C. elegans vha-8(jh135)/bli-6(sc16) egl-19(ad695) unc-24(e318) IV. Show Description
Heterozygotes are WT and segregate WT, Bli Unc, and dead larva. vha-8(jh135) homozygotes are larval lethal.
KK112 C. elegans mel-8(b309) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
mel-8 is a maternal effect lethal. WT heterozygotes (sc13 recessive) segregate WT, MelUncSqt (sick and small), mnC1 dpy-10 unc-52 homozygotes (paralyzed Dpys). Rescued by male mating.
KK332 C. elegans mel-11(it26) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
Heterozygotes are WT and segregate WT, DpyUnc, and UncRol (which give only dead eggs). Maintain by picking WT. mel-11 is not strict. See also WBPaper00002726.
KK338 C. elegans mel-10(it10) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.
KK36 C. elegans unc-4(e120) mel-13(b306) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
Heterozygotes are WT and segregate WT, paralysed DpyUnc and RolUnc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect.
KK96 C. elegans unc-4(e120) mix-1(b285) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
mix-1(b285) is maternal effect lethal. Heterozygotes are WT and segregate WT, Unc-4 worms which are strong Egl, and paralyzed Dpy Uncs.
MT5240 C. elegans unc-5(e53) lin-33(n1043) bli-6(sc16) IV. Show Description
Unc. Vul. Blistered.
MT5242 C. elegans unc-5(e53) bli-6(sc16) IV. Show Description
Blistered Unc.
MT7620 C. elegans lin-45(n2506) unc-24(e138)/bli-6(sc16) unc-24(e138) IV. Show Description
Heterozygotes are Unc and segregate Unc, UncVul, and BliUnc.
NOA4 C. elegans sqt-1(sc13) lin-29(n333) II; lin-12(n137n460) III. Show Description
PJ1156 C. elegans sqt-1(sc13) age-1(mg44) II; ccIs55 V; mgEx499. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. mgEx499 [unc-54p::age-1 + mec-7p::GFP]. Left-hand rollers. Long and thin. Maintain at 16C.
QP218 C. elegans unc-34(e315) dpy-11(e224) rol-9(sc148) V. Show Description
Unc. Dpy. Rol(ts).
QP220 C. elegans unc-60(m35) dpy-11(e224) rol-9(sc148) V. Show Description
Unc. Dpy. Rol(ts).
RA454 C. elegans swsn-1(tm4567)/rol-9(sc148) V. Show Description
Heterozygotes are mostly wild-type but occasionally lack a gonad arm; segregate tm4567 homozygotes (larval/embryonic lethal) and rol-9 homozygotes (Rol). Pick non-Rol to maintain and screen for larval lethals (or by PCR) for tm4567 deletion. Reference: Large EE and Mathies LD (2014 Jan 8). G3, doi: 10.1534/g3.113.009852.
SM1052 C. elegans zen-4(px47) dpy-20(e1282)/bli-6(sc16) unc-24(e138) Show Description
Heterozygotes are WT and segregate WT, Bli Uncs, and dead embryos and L1s (with Pun phenotype).
ST14 C. elegans mua-5(nc14)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. Show Description
Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced.
ST15 C. elegans ncIs2 II; mua-5(nc15)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. Show Description
ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. Neurons visualized with ncIs2.
ST17 C. elegans mua-5(nc17)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. Show Description
Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced.
ST18 C. elegans mua-5(nc18)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. Show Description
Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced.
VC234 C. elegans fre-1(gk173)/unc-51(e369) rol-9(sc148) V. Show Description
Y113G7A.8. Heterozygotes are WT and segregate WT, sterile adults (gk173 homozygotes) and UncRol progeny. Well-balanced; gk173/unc rol heterozygotes occasionally roll and are not necessarily recombinants. Pick WT and check for correct segregation of progeny to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2961 C. elegans ttx-1(ok2889)/unc-51(e369) rol-9(sc148) V. Show Description
Y113G7A.6. Apparent homozygous lethal deletion chromosome balanced by flanking markers. Heterozygotes are WT and segregate WT, Unc-51 Rol-9 homozygotes and ok2889 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TCTCGGGGAGTTGAATTTTG. External right primer: TTTTTCCCGAATTTTTGCAC. Internal left primer: ATGTCTTCCCGCATGAAAAT. Internal right primer: CCAGTGGTCAGAAAGCCAAT. Internal WT amplicon: 1294 bp. Deletion size: 888 bp. Deletion left flank: GTTGTTTTCTAGAAAATCTGAAAATTTTTA. Deletion right flank: TTACGAATATGAAATTTATCAAGGTCTAGG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2971 C. elegans spe-19(ok3428)/unc-51(e369) rol-9(sc148) V. Show Description
Y113G7A.10. Apparent homozygous lethal deletion chromosome balanced by flanking markers. Heterozygotes are WT and segregate WT, Unc-51 Rol-9 homozygotes and ok3428 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CAAAACGTCCAAAGTGTCCC. External right primer: AGTGATTCCCAGATGTCCCA. Internal left primer: TCTCCGAAATGTCCCAGAAA. Internal right primer: CGAAAAATTCGGAAAAATCG. Internal WT amplicon: 1373 bp. Deletion size: 810 bp. Deletion left flank: ACGTCATCCTCCTGAGTTTTTTCGACTTTC. Deletion right flank: TTAAGCCTATTGAAAAGCTCTGAATTGTCC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VT132 C. elegans sqt-1(sc13) lin-29(n333)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
Heterozygotes are slightly shorter than WT and segregate more animals which are slightly shorter than WT, Rollers which are Egl and have a protruding vulva, and DpyUncs.