Caenorhabditis Genetics Center (CGC)

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Strain Species Genotype
BC1548 C. elegans unc-22(s7) let-67(s214)/nT1 IV; +/nT1 V. Show Description
Heterozygotes are WT and segregate WT, Vul and Twitcher Steriles. The sterility can be rescued by male sperm.
DR1215 C. elegans unc-22(s7) let-67(s214) IV/nT1 [let-?(m435)] (IV;V). Show Description
Heterozygotes are WT and segregate WT, dead eggs and Twitchers that arrest as larvae. Maintain by picking WT. Hets twitch in 1% nicotine.
KP4211 C. elegans nuIs214 III. Show Description
nuIs214 [unc-129p::itsn-1::GFP + myo-2p::GFP] III.
OP214 C. elegans unc-119(ed3) III; wgIs214. Show Description
wgIs214 [K12H6.12::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)