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Strain Species Genotype
BC1572 C. elegans +/eT1 III; let-401(s193) dpy-11(e224) unc-42(e270)/eT1 V. Show Description
WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-larval. Maintain by picking WT.
BR5706 C. elegans byIs193; bkIs10. Show Description
byIs193 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. bkIs10 [aex-3p::hTau V337M + myo-2p::GFP]. Worms have severe locomotion defect and slow growth. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603.
BR5944 C. elegans byIs193. Show Description
byIs193 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. F3 pro aggregation fragment is expressed at low levels (line B); hard to detect by western blot. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603.
JIM193 C. elegans ujIs113 II; ujIs193. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujIs193 [nhr-67::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence in fosmid ID#WRM0633cC01 by recombineering. Expression of transgene confirmed by GFP.
OH7310 C. elegans otIs193 syIs63 IV. Show Description
otIs193 [cog-1p::lsy-6hp + rol-6(su1006)]; derived from otEx1450. syIs63 [cog-1::GFP + unc-119(+)]. Egl. Pvul.
TH346 C. elegans unc-119(ed3)III; ddIs193. Show Description
ddIs193 [rabs-5::2xTY1::GFP:: FRT::3xFLAG + Cbr-unc-119(+)]. Pick non-Unc to maintain. References: Sarov M, et al. Cell. 2012 Aug 17;150(4):855-66. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (
JH2814 C. elegans unc-119(ed3) III; axIs1933. Show Description
axIs1933 [(pFM034) GFP::par-2 RNAi-resistant + unc-119(+)].
JH2815 C. elegans unc-119(ed3) III; axIs1934. Show Description
axIs1934 [(pFM035) GFP::par-2 RNAi-resistant [R183-5A] + unc-119(+)].
JH2816 C. elegans unc-119(ed3) III; axIs1935. Show Description
axIs1935 [(pFM036) GFP::par-2 RNAi-resistant [K162A] + unc-119(+)].
JH2817 C. elegans unc-119(ed3) III; axIs1936. Show Description
axIs1936 [(pFM037) GFP::par-2 RNAi-resistant [R163A] + unc-119(+)].