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Strain Species Genotype
BC96 C. elegans unc-22(s16) IV. Show Description
Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine.
CF453 C. elegans muIs16 II; dpy-20(e1282) IV. Show Description
muIs16 [mab-5::GFP + dpy-20(+)]. non-Dpy.
CGC27 C. elegans umnIs16 X. Show Description
umnIs16 [myo-2p::GFP + NeoR, X:15420938 (intergenic)] X. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9.
FC121 C. elegans zzIs16. Show Description
zzIs16 [(pJE3) eff-1p::GFP + rol-6(su1006)]. GFP+ Rollers. Chromosomal insertion of zzEx10. Integration site of zzIs16 not yet mapped, but it is not tightly linked to eff-1 II, unc-119 III, or jcIs1 IV. pJE3 has 7.5 kb of eff-1 upstream sequence inserted into pPD95.75, driving cytoplasmic GFP expression.
HZ111 C. elegans muIs16 II; sor-1(bp_1)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. Show Description
muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage). NOTE: This allele is published as bp1, but has been curated in WormBase as bp_1 in order to differentiate it from the STS marker bP1.
HZ112 C. elegans muIs16 II; sor-1(bp3)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. Show Description
muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage).
HZ66 C. elegans sop-2(bp186) II; him-5(e1490) bxIs16 V. Show Description
bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
HZ67 C. elegans him-5(e1490) bxIs16 V; sor-3(bp185) X. Show Description
bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
KRA345 C. elegans cfi-1(kas16[mNG::AID::cfi-1]) I. Show Description
mNeonGreen and AID tags inserted into endogenous cfi-1 locus. Transgene can be degraded in a background expressing TIR1 co-factor supplemented with auxin. Reference: Li Y, et al. Elife. 2020 Oct 1;9:e59464. doi: 10.7554/eLife.59464. PMID: 33001031.
OC202 C. elegans zyg-1(it25) II; szy-12(bs16) V. Show Description
zyg-1(it25) EMB partially suppressed at 24C.
PHX1812 C. elegans cfi-1(syb1812[cfi-1(delta_enhancer)::mNG::AID]) I. Show Description
A4e enhancer was deleted in endogenously-tagged cfi-1(kas16[mNG::AID::cfi-1]). Expression of cfi-1::mNG::AID is lost in VNC motor neurons. Reference: Li Y, et al. Elife. 2020 Oct 1;9:e59464. doi: 10.7554/eLife.59464. PMID: 33001031.
PHX1856 C. elegans cfi-1(syb1856[cfi-1(mut_COE)::mNG::AID]) I. Show Description
Eight COE motifs in A4e were mutated in endogenously-tagged cfi-1(kas16[mNG::AID::cfi-1]). Expression of cfi-1::mNG::AID is down-regulated in late larval stages and adults. Reference: Li Y, et al. Elife. 2020 Oct 1;9:e59464. doi: 10.7554/eLife.59464. PMID: 33001031.
PJ1077 C. elegans let-60(ga89) IV; lwIs16 X. Show Description
lwIs16 [act-4::lacZ] X. Temperature sensitive gain-of-function allele of ras. At high temperatures worms become Clr. Should also become Muv- not noted. Maintain at 16C.
PJ1192 C. elegans daf-1(m40) IV; iwIs16 X. Show Description
iwIs16 [act-4::lacZ] X. Maintain at 15C. Temperature-sensitive dauer constitutive. 100% dauer formation at 25C; poor dauer recovery at 15C. Maternal effect. Good lacZ expression in spermatheca, but weak in body wall.
PJ1215 C. elegans dbl-1(wk70) V; iwIs16 X. Show Description
iwIs16 [act-4::lacZ] X. Good lacZ expression in spermatheca, but weak in muscle.
VL621 C. elegans unc-119(ed3) III; wwIs16. Show Description
wwIs16 [mir-75p::GFP + unc-119(+)]. Wild type.
YG1036 C. elegans baf-1(gk324) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); zzIs16. Show Description
zzIs16 [(pJE3) eff-1::GFP + rol-6(su1006)]. Heterozygotes are Rollers with pharyngeal GFP signal, and segregate arrested hT2 aneuploids, and non-GFP gk324 homozygotes (Sterile, Roller and Unc). All worms express GFP driven by eff-1 promoter. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal.
BC2409 C. elegans dpy-18(e364)/eT1 [dpy-?] III; let-331(s1608) unc-46(e177)/eT1 [dpy-?] V. Show Description
Heterozygotes are WT and segregate WT, Unc-36???, Sterile DpyUncs, and dead eggs. Maintain by picking WT.
BC2420 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-346(s1619)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpuUncLets. Lethal mid-larval. Maintain by picking WT.
BC2422 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-476(s1621)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adults) and dead eggs. Maintain by picking WT.
BC2440 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) unc-70(s1639)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Uncs, and DpyUnc Lets that hatch.
BC3425 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-460(s1664)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC3684 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-472(s1605)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC3713 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-473(s1602)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. [This strain is acting strangely: it is throwing bent heads. 12/95]
BC3716 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-475(s1606)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult) and dead eggs. Maintain by picking WT.
BC3718 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-407(s1631)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT.
BC3728 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-480(s1607)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult) and dead eggs. Maintain by picking WT.
BC3729 C. elegans dpy-18(e364)/eT1 III; let-478(s1620) let-?(s2169) unc-46(e177)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC3732 C. elegans dpy-18(e364)/eT1 III; unc-46(e177) let-418(s1617)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult; Vulva protrudes; partial rescue at 15C) and dead eggs. Maintain by picking WT.
BC3733 C. elegans dpy-18(e364)/eT1 III; let-481(s1636) unc-46(e177)/eT1 V. Show Description
Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT.
BR5270 C. elegans byIs161. Show Description
byIs161 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. Pan-neuronal over-expression of the F3 pro aggregation fragment of the human Tau protein with K280 deleted (line A). Integration site not mapped. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603.
BR5271 C. elegans byIs162. Show Description
byIs162 [rab-3p::F3(delta)K280 I277P I380P + myo-2p::mCherry]. Pan-neuronal over-expression of the F3 pro aggregation fragment of the human Tau protein with K280 deleted and two isoleucines to proline substitutions in the hexapeptide motifs of the repeat region (line A). These mutations abrogate the aggregation, making this strain an anti-aggregation control for BR5270. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603.
BR5486 C. elegans byIs162; bkIs10. Show Description
byIs162 [rab-3p::F3(delta)K280(I277P)(I308P) + myo-2p::mCherry]. bkIs10 [aex-3p::h4R1NtauV337M + myo-2p::GFP]. Strain co-expressing the F3(delta)K280 anti-aggregation Tau fragment (with two I to P substitutions) and full-length Tau in all neurons. Red and green fluorescence in the pharynx due to the co-injection markers. The F3/2P fragment is not able to nucleate aggregation of full length Tau. bkIs10 contains an integrated transgene encoding the 1N4R isoforms of human tau with the 337M FTDP-17 mutation. Expression is driven by the pan-neuronal promoter aex-3. Over-expression of this transgene results in a pronounced Unc phenotype. Reference: Fatouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603.
BR6563 C. elegans byIs161; bkIs10. Show Description
byIs161 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. bkIs10 [aex-3p::h4R1NtauV337M + myo-2p::GFP]. Strain co-expressing the F3(delta)K280 pro-aggregation Tau fragment and full-length Tau in all neurons. Worms have severe locomotion defect and slow growth. Red and green fluorescence in the pharynx due to the co-injection markers. bkIs10 contains an integrated transgene encoding the 1N4R isoforms of human tau with the 337M FTDP-17 mutation. Expression is driven by the pan-neuronal promoter aex-3. Over-expression of this transgene results in a pronounced Unc phenotype. Derived by additional outcrossing of BR5485. Reference: Fatouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603.
CB5614 C. elegans bus-5(e2701) X. Show Description
Bus (M. nematophilum resistant), bleach-sensitive, non-Bah (Yersinia sensitive). e2701 is an intermediate strength missense allele, S167N (YAASKAA>YAANKAA). Reference: O'Rourke et al (in preparation).
CF1395 C. elegans ceh-20(mu290) III; muIs164. Show Description
muIs164 [tax-4::GFP].
CZ3103 C. elegans juIs167. Show Description
juIs167 [vab-19::GFP + rol-6(su1006)]. Integrated by UV/TMP. Rollers. Received new stock Feb 2005.
FT404 C. elegans ect-2(gk44) II; unc-119(ed3) III; xnIs162. Show Description
xnIs162 [ect-2::GFP + unc-119(+)]. Transgene rescues sterility of ect-2(gk44). Reference: Chan E & Nance J. J Cell Sci. 2013 Apr 1;126(Pt 7):1692-702.
GA905 C. elegans unc-119(ed3) III; pkIs1642. Show Description
pkIs1642 [sir-2.1(+) + unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5.
GA906 C. elegans unc-119(ed3) III; pkIs1641. Show Description
pkIs1641 [unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5.
GR1321 C. elegans tph-1(mg280) cam-1(vs166) II. Show Description
Slow pumping. Egg laying defective. Low frequency of dauer formation. Residual serotonin immunoreactivity, rare and very faint (<1% of NSMs) in most stages, but nearly 100% of CP neurons in adult males show faint to moderate serotonin immunoreactivity. Males mate quite well (E. Hare and C. Loer). Some phenotypic defects originally attributed to mg280 in this strain are likely due to vs166. vs166 is a large deletion (approx. 9.8kb) in the cam-1 gene; flanking sequences are: 5'-gctactggtaaataaggtaa-3' and 5'-atgcttttaaagtttatatt-3' (Edith Myers, personal commnication). See MT15434 for a strain carrying mg280 without the cam-1 mutation.
GS1692 C. elegans unc-4(e120) II; arDp2 (II;f). Show Description
Pick non-Uncs to maintain. arDp2 is derived from mnC1, hence carries dpy-10(e128) and possibly unc-52(e444). arDp2 is a free duplication but does not pass at a high frequency. arDp2 is not stable as a balancer. It is prone to recombination; pick non-Unc and check for segregation of unc-4 and WT in progeny. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
HS1673 C. elegans lin-17(n3091) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); vpIs1 X. Show Description
vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP n3091 homozygotes (Sys Unc Psa). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS1675 C. elegans egl-20(n585) cwn-2(ok895) IV. Show Description
Egl. Unc. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS1680 C. elegans lin-44(n1792) zdIs5 I; cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); mom-2(ne874) V/nT1. Show Description
zdIs5 [mec-4::GFP + lin-15(+)]. Homozygous nT1[qIs51] are inviable. mec-4::GFP is expressed in touch neurons. Heterozygotes are strong Egl Psa GFP+ and segregate dead eggs and non-GFP Unc Egl Psa that give only dead eggs at 25C.
HS169 C. elegans nob-1(os6) III. Show Description
Tail abnormal. Defects in asymmetric T cell division causes Psa (phasmid socket absent) phenotype.
HS1698 C. elegans unc-119(ed3) III; osIs15. Show Description
osIs15 [pie-1p::GFP::apr-1 + unc-119(+)]. Non-Unc. Reference: Cell. 2011 Sep 16;146(6):942-54.
JH2220 C. elegans unc-119(ed3) III; axIs1608. Show Description
axIs1608 [pie-1p::GFP::histone H2B::par-5 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.
JH2221 C. elegans unc-119(ed3) III; axIs1609. Show Description
axIs1609 [pie-1p::GFP::histone H2B::mex-5 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.
JH2223 C. elegans unc-119(ed3) III; axIs1611. Show Description
axIs1611 [pie-1p::GFP::histone H2B::daz-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.