Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal as sterile adult. Maintain by picking WT. [7/94: No longer throwing eT1 Unc-36's. May have picked up a lethal mutation on one of the eT1 chromosomes. Also, appears to be lethal in mid-late larval stage rather than as adult.]

pkIs1582 [let-858::GFP + rol-6(su1006)] V. Homozygous lethal lsm-8 deletion balanced by GFP-marked nT1 translocation. xe17 generated by CRISPR/Cas9-engineered replacement of the gene with a red pharyngeal marker. lsm-8 heterozygotes are wild-type (will roll in this case because of pkIs1582) green & red pharynx, and will segregate rolling heterozygotes (green & red pharynx), arrested nT1[qIs51] aneuploids (only green pharynx), and lsm-8 homozygotes (only red pharynx). Homozygous nT1[qIs51] inviable. Pick rollers with green & red pharynx and check for correct segregation of progeny to maintain. Reference: Mattout A, et al. Nat Cell Biol. 2020 May;22(5):579-590. PMID: 32251399

pkIs1582 [let-858::GFP + rol-6(su1006)]. Rollers.