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Strain Species Genotype
BC160 C. elegans dpy-14(e188) unc-13(e51) let-87(s106)/unc-15(e73) I. Show Description
Heterozygotes are WT and segregate WT, paralyzed Unc and DpyUncLethal. Lethal early larval (L1). Maintain by picking WT.
CF2805 C. elegans dop-2(vs105) V; dop-4(ok1321) dop-1(vs100) dop-3(vs106) X Show Description
Quadruple mutant removing four different dopamine receptor genes.
LX703 C. elegans dop-3(vs106) X. Show Description
292 bp deletion. First 22 bp are: ACTTCCGTATTCCTTCTACTAC. Last 22 bp are: CTTAGCAGTTTCTGATTTTCTG.
LX704 C. elegans dop-2(vs105) V; dop-3(vs106) X. Show Description
LX705 C. elegans dop-1(vs100) dop-3(vs106) X. Show Description
LX734 C. elegans dop-2(vs105) V; dop-1(vs100) dop-3(vs106) X. Show Description
MT11090 C. elegans mcd-1(n3376) II; nIs106 X. Show Description
nIs106 [lin-11::GFP + lin-15(+)] X. Egl, Him. lin-11::GFP expressed in vulva, head neurons, VC neurons. n3376 enhances ced-3(n2427). Reference: (2007) Genetics 175(4)::1719-33.
OP106 C. elegans unc-119(ed3) III; wgIs106. Show Description
wgIs106 [mdl-1::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of mdl-1 coding sequence of fosmid ID#WRM0618dG08 by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
PD9927 C. elegans ced-9(n2812) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); nIs106 X. Show Description
nIs106 [lin-11::GFP + lin-15(+)] X. Homozygous maternal effect lethal ced-9 mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ced-9 homozygotes (maternal effect lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
TG2415 C. elegans vtIs1 dop-2(vs105) V; dop-1(vs100) dop-3(vs106) X. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767.
TG2416 C. elegans vtIs1 dop-2(vs105) V; dop-1(vs100) dop-3(vs106) tsp-17(gt1681) X. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. Reference: Masoudi N, et al. PLoS Genet. 2014 Dec 4;10(12):e1004767.
TH189 C. elegans unc-119(ed3)III; ddIs106. Show Description
ddIs106 [hmg-3::2xTY1::GFP:: FRT::3xFLAG + Cbr-unc-119(+)]. Pick non-Unc to maintain. References: Sarov M, et al. Cell. 2012 Aug 17;150(4):855-66. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)
ZW127 C. elegans zwIs106. Show Description
zwIs106 contains [unc-9p::GFP + lin-15(+)]. Superficially wild-type. Maintain under normal conditions. Described in Altun et al. Dev Dyn 238:1936-50 (2009).
BC10732 C. elegans dpy-5(e907) I; sIs10623. Show Description
sIs10623[rCesF54A5.3a::GFP + pCeh361]. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC10812 C. elegans dpy-5(e907) I; sIs10602. Show Description
sIs10602 contains [rCes K07A9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC10818 C. elegans dpy-5(e907) I; sIs10693. Show Description
sIs10693[rCesY54E2A.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC10819 C. elegans dpy-5(e907) I; sIs10697. Show Description
sIs10697 [rCesZC101.2e::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). sIs10697 [rCesZC101.2e::GFP + pCeh361]
BC10853 C. elegans dpy-5(e907) I; sIs10623. Show Description
sIs10623 [rCesF54A5.3A::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC11207 C. elegans dpy-5(e907) I; sIs10648. Show Description
sIs10648 [rCes Y45F3A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC12415 C. elegans dpy-5(e907) I; sIs10601. Show Description
sIs10601[rCesK08E3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC12416 C. elegans dpy-5(e907) I; sIs10631. Show Description
sIs10631 [rCes Y39B6A.35::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC12417 C. elegans dpy-5(e907) I; sIs10638. Show Description
sIs10638 [rCes Y43F4A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC12419 C. elegans dpy-5(e907) I; sIs10629. Show Description
sIs10629 [rCesY39A1A.15b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
BC1751 C. elegans dpy-18(e364)/eT1 III; let-466(s1063) unc-46(e177)/eT1 V. Show Description
WT strain which segregates WT, Unc-36, dead eggs and DpyUncs. The DpyUncs throw DpyUncs that are Sterile adults. (Mel->Sterile adult in F2.) Maintain by picking WT.
CHS1060 C. elegans srx-12(yum1413) srx-13(yum1414) srx-14(yum1415) IV; srx-33(yum1416) srx-34(yum1417) srx-36(yum1418) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1061 C. elegans tkr-1(yum1419) III; tkr-2(yum1420) tkr-3(yum1421) IV. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1062 C. elegans npr-6(yum1027) npr-7(yum1028) npr-8(yum1029) X. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1063 C. elegans npr-14(yum1423) I; npr-15(yum1424) III; npr-13(yum1422) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1064 C. elegans npr-20(yum1426) II; npr-18(yum1425) npr-21(yum1427) X. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1065 C. elegans npr-26(yum1429) npr-27(yum1430) IV; npr-25(yum1428) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1066 C. elegans srj-1(yum1431) IV; srj-4(yum1432) srj-5(yum1433) srj-6(yum1434) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1067 C. elegans str-96(yum1435) str-97(yum1436) str-99(yum1437) str-101(yum1438) str-102(yum1439) str-103(yum1440) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1068 C. elegans sri-5(yum1445) I; sri-18(yum1441) V; sri-19(yum1442) IV; sri-20(yum1443) sri-21(yum1444) V. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
CHS1069 C. elegans npr-31(yum1446) II; npr-32(yum1447) IV; npr-33(yum1448) X. Show Description
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404.
PS10640 C. elegans cmk-1(sy2277[cmk-1::mKate2::AID*::3xFLAG) IV; syIs875. Show Description
syIs875 [ins-6p::dYFP + ins-6::mCherry + unc-122p::GFP]. cmk-1(sy2277) is a C-terminal knock-in of mKate2::AID::FLAG to be used for conditional degradation of CMK-1 protein. sy2277 is a CRISPR-engineered allele generated using the self-excising cassette (SEC) method (Dickinson et al. 2015, Genetics) with the gRNA sequence 5'-AGCGTGAAAAGCGGGTGTAGNGG-3' (note: NGG not included in the gRNA). syIs875 is an integrated transgene that includes a transcriptional and translational reporter for ins-6 and is marked by GFP in the coelomocytes. dYFP signal can be seen in ASI during reproductive growth and in ASJ (strong) and ASI (weaker) during dauer exit. Reference: Zhang MG, et al. (2024). Available at: https://www.biorxiv.org/content/10.1101/2024.03.20.586022v1 [Accessed 13 August 2024].
RW10595 C. elegans unc-119(ed3) III; zuIs178; stIs10669. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10669 [D1081.8::H1-wCherry::let-858 3' UTR]. May still contain stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)] in the background.
RW10608 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10683. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10683 [T23G5.6::H1-wCherry + unc-119(+)].
RW10609 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10681. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10681 [T23G4.1::H1-wCherry + unc-119(+)].
RW10611 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10692. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10692 [dve-1::H1-wCherry + unc-119(+)].
RW10645 C. elegans unc-119(ed3) III; stIs10645. Show Description
stIs10645 [pax-3p::HIS-24::mCherry + unc-119(+)].
RW10658 C. elegans unc-119(ed3) III; stIs10658. Show Description
stIs10658 [T23H4.2::H1-wCherry + unc-119(+)].
RW10710 C. elegans unc-119(ed3) III; zuIs178; stIs10642. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10642 [F23F12.9::H1-wCherry::let-858 3' UTR]. May still contain stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)] in the background.
RW10711 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10637. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10637 [lin-32::H1-wCherry + unc-119(+)].
RW10722 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10620. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10620 [mnm-2::H1-wCherry + unc-119(+)].
RW10730 C. elegans unc-119(ed3) III; zuIs178; stIs10696. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10696 [F28C6.1::H1-wCherry::his-24::mCherry + unc-119(+)]. May still contain stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)] in the background.
RW10731 C. elegans unc-119(ed3) III; zuIs178; stIs10629. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10629 [F47H4.1::H1-wCherry::let-858 3' UTR]. May still contain stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)] in the background.
RW10740 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10684. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10684 [nhr-67::H1-wCherry + unc-119(+)].
RW10741 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10651. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10651 [ZK185.1::H1-wCherry + unc-119(+)].
RW10742 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10645. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10645 [pax-3::H1-wCherry + unc-119(+)].
RW10745 C. elegans unc-119(ed3) III; zuIs178 V; stIs10024; stIs10698. Show Description
zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. stIs10024 [pie-1::H2B::GFP::pie-1 3' UTR + unc-119(+)]. stIs10698 [sma-9.k::H1-wCherry + unc-119(+)].