WH136 |
C. elegans |
sle-1(oj23) II. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces Mel phenotype.
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WH137 |
C. elegans |
stu-13(oj24) II. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces Mel phenotype.
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WH138 |
C. elegans |
stu-14(oj26) X. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile, slightly Unc animals. L4 shift-up produces Mel phenotype.
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WH140 |
C. elegans |
stu-15(oj28) V. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C sterile Unc animals. L4 shift-up produces Mel phenotype.
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WH142 |
C. elegans |
stu-16(oj30) II. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
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WH143 |
C. elegans |
mett-10(oj32) III. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype. mett-10 was formerly known as stu-18.
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WH144 |
C. elegans |
stu-19(oj33) IV. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are slightly Unc. L4 shift-up produces Mel phenotype.
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WH145 |
C. elegans |
cyk-2(oj34) IV. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
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WH16 |
C. elegans |
stu-9(oj13) II. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are slightly Unc (omega backers). L4 shift-up produces leaky Mel phenotype.
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WH161 |
C. elegans |
stu-17(oj31) V. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
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WH163 |
C. elegans |
nDf29/unc-13(e1091) spd-2(oj29) I. Show Description
Heterozygotes are WT and segregate WT, dead eggs and Uncs. At 25C the Unc Spds are Sterile; at 16C the Unc Spds are fertile but produce mostly dead eggs. Unc Spd animals will exhibit a fully penetrant maternal-effect embryonic lethal phenotype if shifted to 25C at the L4 stage. unc-13 spd-2 homozygotes may be propagated at 16C but may become sick causing immense frustration! See also WBPaper00004200.
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WH170 |
C. elegans |
eff-1(oj55) II. Show Description
Loss of cell fusion in hypodermis (epithelial fusion failures). Viable and fertile as homozygotes. Tail-spike defect in all young larvae, less visible in older larvae and adults. oj55 appears to cause incomplete loss of function, as many cells fuse in postembryonic development. Homozygous males have tail and mating defects. ES-3. Cloned: ORF C26D10.5 encodes a type-I membrane glycoprotein with a single TM domain.
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WH171 |
C. elegans |
eff-1(oj55) II; jcIs1 IV. Show Description
jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Loss of cell fusion in hypodermis (epithelial fusion failures). Viable and fertile as homozygotes. Tail-spike defect in all young larvae, less visible in older larvae and adults. oj55 appears to cause incomplete loss of function, as many cells fuse in postembryonic development. Homozygous males have tail and mating defects. ES=3. Cloned: ORF C26D10.5 encodes a type-I membrane glycoprotein with a single TM domain. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Mohler WA, et al. Curr Biol. 1998 Sep 24;8(19):1087-90.
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WH202 |
C. elegans |
unc-61(n3169) V. Show Description
Poor backward movement. Protrusive vulva. Gonad extrusion. Egg-laying defects. Recessive.
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WH204 |
C. elegans |
unc-119(ed3) III; ojIs1. Show Description
ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain under normal conditions. Reference: Strome et al. (2001) Mol Biol Cell (6):1751-64.
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WH216 |
C. elegans |
sep-1(e2406) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes have myo-2::GFP [qIs48] strongly expressed in the pharynx and are viable at 25C. 100% of sep-1 homozygotes are strongly Sterile Unc at 25C (at 20C, 100% are Sterile but no so Unc). Up to 30% of the homozygotes are Sterile at 16C. hT2[qIs48] homozygotes are dead. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
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WH220 |
C. elegans |
unc-119(ed3) III; ojIs7. Show Description
ojIs7 [zyg-12A::GFP + unc-119(+)].
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WH223 |
C. elegans |
ojIs9. Show Description
ojIs9 [zyg-12(all)::GFP + unc-119(+)].
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WH237 |
C. elegans |
ojIs10. Show Description
ojIs10 [lis-1::GFP + unc-119(+)].
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WH257 |
C. elegans |
unc-119(ed3) III; ojIs5. Show Description
ojIs5 [pie-1p::GFP::dnc-1 + unc-119(+)]
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WH258 |
C. elegans |
unc-119(ed3) III; ojIs57. Show Description
ojIs57 [pie-1::GFP::dnc-2 + unc-119(+)]
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WH259 |
C. elegans |
unc-119(ed3) III; ojIs47. Show Description
ojIs47 [arp-1::GFP + unc-119(+)].
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WH276 |
C. elegans |
unc-119(ed3) III; ojIs13. Show Description
ojIs13 [zyg-12B/C::GFP + unc-119(+)].
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WH279 |
C. elegans |
unc-119(ed3) III; ojIs12. Show Description
ojIs12 [cyk-4::GFP + unc-119(+)]. Strain is not very healthy. Described as integratred array but appears to segregate Uncs. Maintain at 15-20C.
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WH280 |
C. elegans |
unc-119(ed3) III; ojEx38. Show Description
ojEx38 [pie-1::GFP::cmd-1 + unc-119(+)]. Reference: Batchelder et al. (2007) FEBS Lett 581(22):4337-41.
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WH32 |
C. elegans |
stu-10(oj14) I. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are Unc (slow omega backers). L4 shift-up produces leaky Mel phenotype.
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WH327 |
C. elegans |
unc-119(ed3) III; ojIs23. Show Description
ojIs23 [pie-1p::GFP::C34B2.10].
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WH33 |
C. elegans |
stu-11(oj18). Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces leaky Mel phenotype. Unmapped.
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WH342 |
C. elegans |
unc-119(ed3) III; ojIs31. Show Description
ojIs31 [pie-1p::spd-3::GFP + unc-119(+)]. ojIs31 rescues spd-3(oj35) lethality at 25 C. Maintain at 25 C. Reference: Dinkelmann MV, et al., Genetics. 2007 Nov;177(3):1609-20.
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WH346 |
C. elegans |
unc-119(ed3) III; ojIs34. Show Description
ojIs34 [GFP::car-1 + unc-119(+)]. N'-tagged GFP::CAR-1 (Y18D10A.17) fusion. Labels P-granules and small cytoplasmic puncta in all cells. Bombardment with pNL1.6::GFP::unc-119 (pfj-1::pie-1 promoter driving GFP::Y18D10A.17 (N-terminal) with unc-119 rescuing fragment).
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WH347 |
C. elegans |
unc-119(ed3) III; ojIs35. Show Description
ojIs35 [pie-1::GFP::rab-11.1 + unc-119(+)].
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WH351 |
C. elegans |
unc-119(ed3) III; ojIs37. Show Description
ojIs37 [pie-1p::GFP::ugtp-1 + unc-119(+)]. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.
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WH371 |
C. elegans |
unc-119(ed3) III; ojIs50. Show Description
ojIs50 [pie-1p::GFP::air-2 + unc-119(+)]. Wild type looking worms with GFP expression in early embryos. GFP can be seen on stereo microscope in complete darkness with sharp eyes.
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WH377 |
C. elegans |
car-1(tm1753) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT and GFP+. car-1 homozygotes are have no GFP and produce embryos with cytokinesis defect (embryonic lethal). Some recombination.
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WH408 |
C. elegans |
sep-1(e2406) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Homozygous embryonic lethal mutation balanced by bli-4- and GFP-marked translocation. Maintain at 15 C. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP e2406 homozygotes (embryonic arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Bembenek J, et al. Curr Biol (2010) Feb 9;20(3):259-64.
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WH416 |
C. elegans |
unc-119(ed3) ojIs58 III. Show Description
ojIs58 [pie-1p::sep-1::GFP + unc-119(+)] III. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.
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WH438 |
C. elegans |
unc-119(ed3) III; ojEx64. Show Description
ojEx64 [pie-1p::sep-1::GFP + unc-119(+)]. Pick wild-type. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.
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WH468 |
C. elegans |
sep-1(e2406) I/hT2 (I;III); ruIs32 III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Homozygous embryonic lethal mutation balanced by bli-4- and GFP-marked translocation. Maintain at 15 C. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP e2406 homozygotes (embryonic arrest). Homozygous hT2 [bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Bembenek J, et al. Curr Biol (2010) Feb 9;20(3):259-64.
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WH485 |
C. elegans |
sep-1(e2406) I/hT2 (I;III); ojIs58 III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
ojIs58 [pie-1p::sep-1::GFP + unc-119(+)] III. Homozygous embryonic lethal mutation balanced by bli-4- and GFP-marked translocation. Maintain at 15 C. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP e2406 homozygotes (embryonic arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Bembenek J, et al. Curr Biol (2010) Feb 9;20(3):259-64.
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WH49 |
C. elegans |
stu-8(oj1) X. Show Description
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
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WH497 |
C. elegans |
unc-119(ed3) III; ojIs69. Show Description
ojIs69 [pie-1::mGFP::chin-1 + unc-119(+)]. N-terminal fusion of mGFP to sole predicted BE0003N10.2 ORF. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77.
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WH498 |
C. elegans |
unc-119(ed3) III; ojEx83. Show Description
ojEx83 [pie-1p::mCherry::chin-1a + unc-119(+)]. Maintain by picking wild-type. Check occasionally for maintained mCherry expression.
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WH515 |
C. elegans |
chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III. Show Description
Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
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WH517 |
C. elegans |
ojIs40. Show Description
ojIs40 [pie-1::mGFP::wsp-1(G-protein binding domain) + unc-119(+)]. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77.
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WH527 |
C. elegans |
cgef-1(gk261) X; ojIs40. Show Description
ojIs40 [wsp-1(G-protein-Binding-Domain)::GFP + unc-119(+)]. Maintain under normal condition. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
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WH528 |
C. elegans |
cgef-1(gk261) X; ojIs26. Show Description
ojIs26 [GFP::nmy-2 + unc-119(+)]. Maintain under normal conditions. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
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WH529 |
C. elegans |
cgef-1(gk261) X; ddIs?. Show Description
ddIs? [pie-1p::GFP::par-6 + unc-119(+)]. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
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WH530 |
C. elegans |
cgef-1(gk261) X; itIs153. Show Description
itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. Maintain at 24 degrees; sick at 25 C, GFP lost at 20C. itIs153 is an integrated derivitive of axEx1094. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
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WH532 |
C. elegans |
chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ddIs?. Show Description
ddIs? [pie-1p::GFP::par-6 + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
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WH533 |
C. elegans |
chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ddIs?. Show Description
ddIs? [pie-1p::mCherry::par-6 + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
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