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Strain Species Genotype
NL3321 C. elegans sid-1(pk3321) V. Show Description
Resistant to feeding dsRNA but sensitive to dsRNA injection. PKA rsd-8.
TU3401 C. elegans sid-1(pk3321) V; uIs69 V. Show Description
uIs69 [pCFJ90 (myo-2p::mCherry) + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. Maintain 15-20 degrees. [NOTE: uIs69 is closely linked and maps to the right of dpy-11. (C. Loer 08/2011) See strains LC105 and LC108.] Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3568 C. elegans sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. Show Description
uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3595 C. elegans sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. Show Description
uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette.
TU3596 C. elegans sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. Show Description
Him. Enhanced RNAi background. Maintain under normal conditions.