More Fields
Strain Species Genotype
RB525 C. elegans pgl-3(ok257) V. Show Description
C18G1.4a. Superficially wild type. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
SS608 C. elegans pgl-3(bn104) V. Show Description
Fertile at all temperatures.
JH3230 C. elegans pgl-3(bn104) V; axIs2076. Show Description
axIs2076 [meg-3p::GFP::meg-3::meg-3 3'UTR + unc-119(+)]. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591.
JH3657 C. elegans pgl-3(ax4300[pgl-3::mCherry]) fog-2(q71) V. Show Description
mCherry inserted at C terminus of endogenous pgl-3 locus. fog-2(q71) is male/female strain. Maintain by mating. XX animals are female. XO animals are WT males. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226.
SS609 C. elegans pgl-3(bn104) dpy-11(e224) V. Show Description
Dpy. Fertile at all temperatures.
SS618 C. elegans pgl-1(ct131) him-3(e1147) IV; pgl-3(bn104) V. Show Description
Throws males. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS731 C. elegans pgl-2(bn123) III; pgl-3(bn104) dpy-11(e224) V. Show Description
Dpy. Fertile at all temperatures.
SS615 C. elegans pgl-1(bn101) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. Show Description
Dpy Unc. Temperature sensitive. Maintain at 15C. Grows very slowly.
SS617 C. elegans pgl-1(ct131) him-3(e1147) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. Show Description
Throws males. Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
SS733 C. elegans pgl-2(bn123) III; pgl-1(bn101) unc-24(e138) IV; pgl-3(bn104) dpy-11(e224) V. Show Description
Dpy. Unc. Mostly sterile at all temperatures, but a small fraction are fertile at low temperatures. Maintain at 15C.
JH2017 C. elegans unc-119(ed3) III; axIs1464. Show Description
axIs1464 [pie-1p::GFP::pgl-3 ORF::pgl-3 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.
JH2349 C. elegans unc-119(ed3) III; axIs1696. Show Description
axIs1696 [pie-1p::GFP::histone H2B:pgl-3 3'utr , unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.
JH3019 C. elegans unc-119(ed3) III; axIs2076; axIs2077. Show Description
axIs2076 [meg-3p::GFP::meg-3::meg-3 3'UTR + unc-119(+)]. axIs2077 [pie-1p::GFP::pgl-3::pie-1 3'UTR]. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591.
JH3553 C. elegans meg-3(ax4503[del(545-862)::OLLAS]) meg-4(ax4504) X. Show Description
20% maternal effect sterility on average, RNAi insensitivity, MEG-3 does not form cytoplasmic gradient. meg-3(ax4503) is an-frame deletion in the endogenous meg-3 locus removing amino acids 545-862; MEG-3 does not form a cytoplasmic gradient but does still form granules in the early embryos and co-localizes with PGL-3 . References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570.