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Strain Species Genotype
RB1013 C. elegans pax-2(ok935) IV. Show Description
K06B9.5. Homozygous. Outer Left Sequence: GGGCAGAACACGAATTTTTC. Outer Right Sequence: GCACATCCCTGGTTGAAACT. Inner Left Sequence: CGCAAATTTGGTCCTGAAAT. Inner Right Sequence: CTCCGCCTACTGTTAGCTCG. Inner Primer WT PCR product: 2959. Deletion size: 1620 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
RB1019 C. elegans pax-2(ok946) IV. Show Description
K06B9.5. Homozygous. Outer Left Sequence: GGGCAGAACACGAATTTTTC. Outer Right Sequence: GCACATCCCTGGTTGAAACT. Inner Left Sequence: CGCAAATTTGGTCCTGAAAT. Inner Right Sequence: CTCCGCCTACTGTTAGCTCG. Inner Primer WT PCR product: 2959. Deletion size: 712 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
VC2438 C. elegans pax-2(ok3078) IV. Show Description
K06B9.5. External left primer: AGGCTAATGAAACGTGCCAA. External right primer: AATTTTCGAGTCGTTGGTCG. Internal left primer: ACAGAGAAATGGCGAGTTGC. Internal right primer: CACGTGGGTAATGTGGTACG. Internal WT amplicon: 1183 bp. Deletion size: 527 bp. Deletion left flank: AACTAAAAAGTGTACTTTATTGAGATTATA. Deletion right flank: GTGTTCTCCGACAAGTCCAGTAGGATAGAC. Insertion Sequence: GTGTACTTTATTGAGATTATA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
OP741 C. elegans unc-119(tm4063) III; wgIs741. Show Description
wgIs741 [npax-2::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).