More Fields
Strain Species Genotype
KK1262 C. elegans par-1(it324[par-1::GFP::par-1 exon11a]) V. Show Description
Superficially wild-type. Made in N2 background.
KK822 C. elegans par-1(zu310) V. Show Description
Temperature-sensitive maternal effect lethal. Maintain at 15C. At 25C embryos show polarity defects and fail to hatch. Reference: Morton DG, Hoose WA, Kemphues KJ. Genetics. 2012 Aug 10.
RB1625 C. elegans par-1(ok2001) V. Show Description
H39E23.1. Homozygous. Outer Left Sequence: ATGTCGGCGGCTGATATTAC. Outer Right Sequence: ACGGAGTCAATTTTTGACGG. Inner Left Sequence: GAGTGAACGAGAAAAAGGCG. Inner Right Sequence: CCACGTCAATCCGAGAGTTT. Inner Primer PCR Length: 2534 bp. Deletion Size: 594 bp. Deletion left flank: ACACTTTTCGCTTCGGGATCAGAGATTCCT. Deletion right flank: TCATTCGATGCTGACTGCTTGCGGGGCCAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
KK288 C. elegans sqt-3(sc8) par-1(b274) IV/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Unc, RolPar (adult homozygotes lay eggs that don't hatch) and dead eggs. nT1[unc-?(n754) let-?] is dominant Unc and recessive lethal. Strict maternal effect. sc8 previously called rol-4(sc8).
ATD1 C. elegans unc-119(ed3) III; sqt-3(sc8) par-1(b274) IV/nT1[unc-?(n754) let-?] (IV;V); zuIs45 V. Show Description
zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Balanced heterozygotes are Unc and segregate Unc (heterozygotes), Rol Par (sqt-3 par-1 homozygotes; maternal effect lethal), and dead eggs (nT1 homozygotes). NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and KK288. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231.
TH205 C. elegans unc-119(ed3) III; ddEx120. Show Description
ddEx120 [cpar-1::TY1::EGFP::3xFLAG(92C12) + Cbr-unc-119(+)]. Pick non-Unc to maintain. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Cell. 2012 Aug 17;150(4):855-66. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)