EU91 |
C. elegans |
apx-1(or3) V/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Unc and wild type animals which give only dead progeny.
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AMH25 |
C. elegans |
dhc-1(or352) I; sosIs5. Show Description
sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Maintain at 15 degrees. Temperature-sensitive embryonic lethal. 100% dead embryos at 25 degrees. Small, posteriorly displaced first mitotic spindle with defective chomosome segregation and cytokinesis.
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ATD2 |
C. elegans |
par-2(or373) unc-119(ed3) III; zuIs45 V. Show Description
zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Temperature-sensitive maternal-effect lethal. Maintain at 15C. NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and EU822. Unknown if unc-119(ed3) is still present or homozygous in background. NOTE: this strain was originally described as heterozygous for lin-2(e1309), but lin-2 has been lost; this strain is now homozygous wild-type for lin-2. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231.
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EU1008 |
C. elegans |
apo-5(or358) I. Show Description
Temperature sensitive, embryonic lethal mutant. 98% of embryos produced by homoygous mothers hatch at 15C; 0% produced at 26C hatch (but some viability at 25C). Mutation is recessive at 26C. First mitotic spindle in one-cell zygote is mis-positioned, often abnormally near the posterior pole; some chromosome segregation defects.
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EU1133 |
C. elegans |
apo-5(or358) ruIs32 III Show Description
ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. apo-5(or358) is temperature-sensitive embryonic lethal. 100% dead embryos when L4 larvae are shifted to 26.6C. A small percentage of embryos survive form L4 larvae shifted to 25-26C. or358 appears to be semi-dominant; L4 or358/+ heterozygotes shifted to 26C produce ~20% embryonic lethality. Maintain at 15C. References: Encalada SE, et al., Mol Biol Cell. 2005 Mar;16(3):1056-70. Strome S, et al. Mol Biol Cell. 2001 Jun;12(6):1751-64.
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EU1135 |
C. elegans |
tba-1(or346) I. Show Description
Dominant, conditional, maternal-effect. At 15C, almost all embryos hatch from or346/+ and or346/or346 mothers. At25C, about 85% of embryos die from or346/+ mothers and about 100% of embryos die from or346/or346 mothers.
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EU1386 |
C. elegans |
dhc-1(or352ts) Show Description
Temperature-sensitive embryonic lethal. 100% dead embryos at 25 degrees. Maintain at 15 degrees. Small, posteriorly displaced first mitotic spindle with defective chomosome segregation and cytokinesis.
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EU396 |
C. elegans |
cyk-1(or36) unc-36(e251)/qC1 [dpy-19(e1259) glp-1(q339)] III. Show Description
Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs.
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EU407 |
C. elegans |
mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(e1489) IV; lin-2(e1309) X. Show Description
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EU414 |
C. elegans |
unc-13(e1091) mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Show Description
Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or39 is a recessive maternal-effect embryonic lethal.
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EU415 |
C. elegans |
cyk-1(or36) unc-36(e251)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-8(e1489) IV. Show Description
Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs at all temperatures. Throws males.
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EU417 |
C. elegans |
mom-2(or33) V/nT1 [unc-?(n754) let-?] (IV;V). Show Description
mom-2(or33) is a recessive, non-conditional maternal-effect embryonic lethal. Intermediate strength allele; missense mutation near C-terminus: C321Y. 50% of mutant embryos lack gut. Heterozygotes are Unc.
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EU423 |
C. elegans |
dpy-5(e61) mom-4(or39)/hT1 I; mom-2(or42) unc-42(e270)/hT1 V. Show Description
Heterozygotes are WT.
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EU446 |
C. elegans |
unc-13(e1091) mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Show Description
Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or39 is a recessive maternal-effect embryonic lethal.
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EU447 |
C. elegans |
unc-13(e1091) mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(e1489) IV. Show Description
Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or39 is a recessive maternal-effect embryonic lethal. Throws males.
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EU822 |
C. elegans |
par-2(or373) III; lin-2(e1309) X. Show Description
Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644.
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EU855 |
C. elegans |
mom-2(or309) V/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Uncs, WT which give only dead eggs, and dead eggs. or309 is a deletion allele: removes exon 4 to 3' end and leave about 100 N-terminal amino acids.
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EU858 |
C. elegans |
tbb-2(or362) III. Show Description
Conditional, semi-dominant, maternal-effect, embryonic-lethal mutation. Once-cell stage mitotic embryos have short astral microtubules and a mispositioned mitotic spindle. Maintain at 15C.
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EU863 |
C. elegans |
div-(or361) him-8(e1489) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU867 |
C. elegans |
div-(or389) II. Show Description
Temperature sensitive. Maintain at 15C.
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EU868 |
C. elegans |
div-(or361) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU869 |
C. elegans |
div-(or389) II; him-8(e1489) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU872 |
C. elegans |
div-(or366) I; him-8(e1489) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU873 |
C. elegans |
div-(or310) I. Show Description
Temperature sensitive. Maintain at 15C.
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EU878 |
C. elegans |
div-(or335) I; him-8(e1489) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU879 |
C. elegans |
div-1(or345) III; him-8(e1489) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU880 |
C. elegans |
div-1(or345) III. Show Description
Temperature sensitive. Maintain at 15C.
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EU882 |
C. elegans |
div-(or310) I; him-8(e1489) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU883 |
C. elegans |
div-(or364) III; him-8(e1489) IV. Show Description
Temperature sensitive. Maintain at 15C.
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EU888 |
C. elegans |
div-(or366) I. Show Description
Temperature sensitive. Maintain at 15C.
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EU920 |
C. elegans |
lit-1(or393) III; him-8(e1489) IV. Show Description
Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644.
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HS1795 |
C. elegans |
dsh-2(or302) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. Show Description
Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) mig-5(tm2639) homozygotes (Sys). Pick WT GFP+ animals and check for correct segregation of progeny to maintain.
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HS2690 |
C. elegans |
dsh-2(or302) dsh-1(ok1445)/mIn1 [dpy-10(e128) mIs14] II. Show Description
Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) dsh-1(ok1445) homozygotes (Sys Unc). Pick WT GFP+ animals and check for correct segregation of progeny to maintain.
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HS2725 |
C. elegans |
dsh-2(or302) dsh-1(ok1445) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. Show Description
Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and non-GFP triple homozygotes (mostly Emb with a few animals surviving to early L1). Pick WT GFP+ animals and check for correct segregation of progeny to maintain.
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HY601 |
C. elegans |
mat-3(or344) III. Show Description
Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-4(or344).
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HY607 |
C. elegans |
apc-1(or319) II. Show Description
Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 24C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2.
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HY618 |
C. elegans |
apc-1(or374) II; him-5(e1490) V. Show Description
Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2..
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HY619 |
C. elegans |
apc-1(or385) II; him-5(e1490) V. Show Description
Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2.
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NG3124 |
C. elegans |
dsh-2(or302)/mIn1 [mIs14 dpy-10(e128)] II. Show Description
mIs14 [myo-2p::GFP + pes-10p::GFP]. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP or302 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. or302 homozygotes are GFP- and are Emb, ABar and have EMS spindle misalignment. or302 is a deletion starting at 503 and ending at 1578 of C27A2.6.
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