F26F4.7. Homozygous. Outer Left Sequence: TTGGGGTAACCTCCTCACTG. Outer Right Sequence: TTGGCACAGAACCACTACCA. Inner Left Sequence: GGATATGGGGTCTGTGATGG. Inner Right Sequence: TGTGAATTGGAAACACCGAA. Inner Primer WT PCR product: 2808. Deletion size: 1496 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Pick wild-type GFP+ to maintain. Heterozygotes are WT with GFP+ pharynx, and segregate Dpy GFP+ mIn1 homozygotes, and GFP- low viability non-Egl hermaphrodites. Viability of first generation nDf50 homozygotes (GFP-) segregated from balanced heterozygotes is rescued by maternal contribution of mir-35-41 from balancer. Second generation GFP- animals are low viability. Reference: McJunkin K, Ambros V. Genes Dev. 2017 Feb 15;31(4):422-437.